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Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities

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Autor(es):
Alessi, Anna M. [1] ; Bird, Susannah M. [1] ; Bennett, Joseph P. [1] ; Oates, Nicola C. [1] ; Li, Yi [1] ; Dowle, Adam A. [2] ; Polikarpov, Igor [3] ; Young, J. Peter W. [4] ; McQueen-Mason, Simon J. [1] ; Bruce, Neil C. [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ York, Dept Biol, Ctr Novel Agr Prod, York YO10 5DD, N Yorkshire - England
[2] Univ York, Dept Biol, Biosci Technol Facil, York YO10 5DD, N Yorkshire - England
[3] Univ Sao Paulo, Inst Fis Sao Carlos, Grp Biotecnol Mol, Sao Carlos, SP - Brazil
[4] Univ York, Dept Biol, York YO10 5DD, N Yorkshire - England
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 7, MAY 24 2017.
Citações Web of Science: 10
Resumo

Microbial communities metabolize plant biomass using secreted enzymes; however, identifying extracellular proteins tightly bound to insoluble lignocellulose in these microbiomes presents a challenge, as the rigorous extraction required to elute these proteins also lyses the microbes associated with the plant biomass releasing intracellular proteins that contaminate the metasecretome. Here we describe a technique for targeting the extracellular proteome, which was used to compare the metasecretome and meta-surface-proteome of two lignocellulose-degrading communities grown on wheat straw and rice straw. A combination of mass spectrometry-based proteomics coupled with metatranscriptomics enabled the identification of a unique secretome pool from these lignocellulose-degrading communities. This method enabled us to efficiently discriminate the extracellular proteins from the intracellular proteins by improving detection of actively secreted and transmembrane proteins. In addition to the expected carbohydrate active enzymes, our new method reveals a large number of unknown proteins, supporting the notion that there are major gaps in our understanding of how microbial communities degrade lignocellulosic substrates. (AU)

Processo FAPESP: 10/52362-5 - Targeted analysis of microbial lignocellulolytic secretomes: a new approach to enzyme discovery
Beneficiário:Igor Polikarpov
Linha de fomento: Auxílio à Pesquisa - Regular