The Antimicrobial Peptide LL-37 as a Possible Adjunct for the Proliferation and Differentiation of Dental Pulp Stem Cells

Autor(es)

Moreira Milhan, Noala Vicensoto ^[1] ; de Barros, Patricia Pimentel ^[1] ; de Lima Zutin, Elis Andrade ^[1] ; de Oliveira, Felipe Eduardo ^[1] ; Ribeiro Camargo, Carlos Henrique ^[2] ; Afonso Camargo, Samira Esteves ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Inst Sci & Technol, Dept Restorat Dent, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 43, n. 12, p. 2048-2053, DEC 2017.
• Citações Web of Science: 3

Resumo

Introduction: This study evaluated the biocompatibility of 5 and 10 mu g/mL LL-37 in vitro and its effect on the differentiation of human dental pulp stem cells (DPSCs) into odontoblast-like cells. Methods: Cell viability, genotoxicity, nitric oxide production, cell cycle, dentine sialophosphoprotein (DSPP) production, and DSPP gene expression. Results: Concentrations of 5 and 10 mu g/mL of LL-37 were not cytotoxic and generally increased cell viability, especially on the third day (P < .05). The tested concentrations did not induce genotoxicity (P < .05). LL-37 did not significantly alter nitrite production at either concentration. Cell cycle analysis revealed that 10 1, mu g/mL of LL-37 arrested cells in G0/G1 (P < .05). The control group exhibited higher numbers of cells in other phases of the cell cycle (P < .05). The expression of the DSPP protein and gene was also higher in the 10 mu g/mL of LL-37 group (P < .05). Conclusions: These results demonstrated that LL-37 was biocompatible at these concentrations and increased the number of viable cells, especially during the initial period. The 10 mu g/mL concentration arrested the cell cycle and increased expression of the DSPP protein and gene, which indicates that this peptide contributes to odontoblastic differentiation. (AU)

• Processo FAPESP: 15/13231-6 - Avaliação da biocompatibilidade de scaffolds de nanofibras contendo LL-37 e do potencial de mineralização de células DPSC sensibilizadas por LPS em contato com estes scaffolds.
• Beneficiário: Samira Esteves Afonso Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular