Rhodococcus psychrotolerans sp nov., isolated from rhizosphere of Deschampsia antarctica

Autor(es)

Silva, Leonardo Jose ^{[1, 2]} ; Souza, Danilo Tosta ^[2] ; Genuario, Diego Bonaldo ^[2] ; Vargas Hoyos, Harold Alexander ^{[1, 2]} ; Santos, Suikinai Nobre ^[2] ; Rosa, Luiz Henrique ^[3] ; Zucchi, Tiago Domingues ^[4] ; Melo, Itamar Soares ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Padua Dias Ave 11, BR-13418900 Sao Paulo - Brazil

^[2] EMBRAPA Environm, Lab Environm Microbiol, Brazilian Agr Res Corp, SP 340 Highway, Km 127-5, BR-13820000 Sao Paulo - Brazil

^[3] Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, Presidente Antonio Carlos Ave 6627, BR-31270901 Belo Horizonte, MG - Brazil

^[4] Agrivalle, Agr Biotechnol, Tranquillo Giannini Ave 1090, BR-13329600 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY; v. 111, n. 4, p. 629-636, APR 2018.
• Citações Web of Science: 3

Resumo

A novel actinobacterium, designated strain CMAA 1533(T), was isolated from the rhizosphere of Deschampsia antarctica collected at King George Island, Antarctic Peninsula. Strain CMAA 1533(T) was found to grow over a wide range of temperatures (4-28 A degrees C) and pH (4-10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533(T) belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus Rhodococcus. Sequence similarity calculations indicated that the novel strain is closely related to Rhodococcus degradans CCM 4446(T), Rhodococcus erythropolis NBRC 15567(T) and Rhodococcus triatomae DSM 44892(T) (ae<currency> 96.9%). The organism was found to contain meso-diaminopimelic acid, galactose and arabinose in whole cell hydrolysates. Its predominant isoprenologue was identified as MK-8(H-2) and the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The major fatty acids were identified as Summed feature (C-16:1 omega 6c and/or C-16:1 omega 7c), C-16:0, C-18:1 omega 9c and 10-methyl C-18:0. The G+C content of genomic DNA was determined to be 65.5 mol%. Unlike the closely related type strains, CMAA 1533(T) can grow at 4 A degrees C but not at 37 A degrees C and was able to utilise adonitol and galactose as sole carbon sources. Based on phylogenetic, chemotaxonomic and physiological data, it is concluded that strain CMAA 1533(T) (= NRRL B-65465(T) = DSM 104532(T)) represents a new species of the genus Rhodococcus, for which the name Rhodococcus psychrotolerans sp. nov. is proposed. (AU)

• Processo FAPESP: 13/25505-8 - Diversidade microbiana: a importância da exploração de novas fontes de biodiversidade
• Beneficiário: Danilo Tosta Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/26131-7 - Análise do microbioma do Rio Amazonas e seus afluentes: biogeografia e sua contribuição na fixação do N2
• Beneficiário: Diego Bonaldo Genuário
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado