Time-dependent regulation of morphological changes and cartilage differentiation markers in the mouse pubic symphysis during pregnancy and postpartum recovery

Autor(es)

Castelucci, Bianca Gazieri ^[1] ; Consonni, Silvio Roberto ^[1] ; Rosa, Viviane Souza ^[1] ; Sensiate, Lucimara Aparecida ^[1] ; Rugno Delatti, Paula Cristina ^[1] ; Alvares, Lucia Elvira ^[1] ; Joazeiro, Paulo Pinto ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 4 APR 5 2018.
• Citações Web of Science: 1

Resumo

Animal models commonly serve as a bridge between in vitro experiments and clinical applications; however, few physiological processes in adult animals are sufficient to serve as proof-of-concept models for cartilage regeneration. Intriguingly, some rodents, such as young adult mice, undergo physiological connective tissue modifications to birth canal elements such as the pubic symphysis during pregnancy; therefore, we investigated whether the differential expression of cartilage differentiation markers is associated with cartilaginous tissue morphological modifications during these changes. Our results showed that osteochondral progenitor cells expressing Runx2, Sox9, Col2a1 and Dcx at the non-pregnant pubic symphysis proliferated and differentiated throughout pregnancy, giving rise to a complex osteoligamentous junction that attached the interpubic ligament to the pubic bones until labour occurred. After delivery, the recovery of pubic symphysis cartilaginous tissues was improved by the time-dependent expression of these chondrocytic lineage markers at the osteoligamentous junction. This process potentially recapitulates embryologic chondrocytic differentiation to successfully recover hyaline cartilaginous pads at 10 days postpartum. Therefore, we propose that this physiological phenomenon represents a proof-of-concept model for investigating the mechanisms involved in cartilage restoration in adult animals. (AU)

• Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
• Beneficiário: Hernandes Faustino de Carvalho
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/25038-8 - Influencia da Depleção de Macrófagos na Remodelação da Sínfise Púbica de camundongos C57BL6, Durante o final da Prenhês e o Pós-parto.
• Beneficiário: Bianca Gazieri Castelucci
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/23616-2 - Caracterização de estados de ativação de monócitos e macrófagos presentes no remodelamento da sínfise púbica do camundongo, durante o final da prenhez e o pós-parto.
• Beneficiário: Paulo Pinto Joazeiro
• Linha de fomento: Auxílio à Pesquisa - Regular