Validation of an Enzyme Immunoassay for Measuring Fecal Cortisol Metabolites of Bearded (Sapajus libidinosus) and Black (Sapajus nigritus) Capuchins

Autor(es)

Mendonca-Furtado, Olivia ^[1] ; Izar, Patricia ^[1] ; Palme, Rupert ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Psychol, Sao Paulo - Brazil

^[2] Univ Vet Med, Dept Biomed Sci, Vienna - Austria

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF PRIMATOLOGY; v. 38, n. 6, p. 1002-1016, DEC 2017.
• Citações Web of Science: 3

Resumo

Fecal steroid analysis is a powerful noninvasive tool for behavioral endocrinology, but enzyme immunoassays (EIAs) require experimental validation before they are applied. We conducted a physiological validation of an in-house EIA measuring fecal cortisol metabolites (FCMs) by performing an adrenocorticotrophic hormone (ACTH) challenge, dexamethasone suppression, and control saline solution injection experiment with six male and five female bearded capuchins (Sapajus libidinosus). We also took advantage of a presumably stressful incident to perform a biological validation for females. In addition, we conducted high-performance liquid chromatography (HPLC) immunograms to characterize the FCMs measured in both sexes of bearded capuchin, and in a closely related species (S. nigritus, black capuchin monkeys). Male and female S. libidinosus showed FCM peaks after ACTH injections, and females also showed FCM peaks in the biological validation. Three of four individuals (two males and one female) had an FCM peak shortly after injection of dexamethasone and both sexes then showed prolonged low FCM levels after dexamethasone injection. We observed no effects of saline solution injections. The time to peak FCM excretion after ACTH injection or a stressful incident varied 1.5-8.5 h. HPLC results revealed no differences in FCM profiles between sexes or species and suggest that the EIA can also be used in male and female S. nigritus. Our results validate an in-house EIA for both sexes of S. libidinosus but show large individual variation in FCM excretion, which highlights the need for carefully planned feces collection in endocrinologial research. (AU)

• Processo FAPESP: 08/52293-3 - Medidas de metabolitos de cortisol em macacos-prego (genero cebus): analise comparativa entre populacoes para investigacao de fatores estressores.
• Beneficiário: Olívia de Mendonça Furtado Pimenta
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/55684-3 - Variabilidade de comportamento social de macacos-prego (gênero Cebus): análise comparativa entre populações para investigação de correlatos fisiológicos
• Beneficiário: Patrícia Izar Mauro
• Modalidade de apoio: Auxílio à Pesquisa - Regular