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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The role of androgens on periodontal repair in female rats

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Autor(es):
Steffens, Joao Paulo [1] ; Leal Santana, Luis Carlos [2] ; Paiva Pitombo, Jonleno Coutinho [2] ; Ribeiro, Daniel Olivio [2] ; Costa Albaricci, Maria Carolina [2] ; Cubas Warnavin, Stephanie von Stein [1] ; Kantarci, Alpdogan [3] ; Spolidorio, Luis Carlos [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Parana UFPR, Dept Stomatol, Curitiba, PR - Brazil
[2] Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Physiol & Pathol, Araraquara, SP - Brazil
[3] Forsyth Inst, Dept Appl Oral Sci, Cambridge, MA - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Periodontology; v. 89, n. 4, p. 486-495, APR 2018.
Citações Web of Science: 1
Resumo

Background: Testosterone replacement enhances cognitive function and musculoskeletal health in postmenopausal women. However, the biological role of testosterone on inflammation and bone metabolism in females is not well understood. Our objective was to measure the impact of androgens and their receptors on periodontal tissues during periodontal repair in female rats. Methods: Seventy female Holtzman rats were divided into seven groups (n = 10/group): negative control; repair control; androgen receptor antagonist (flutamide, 50 mg/kg, every other day); estrogen receptor antagonist (fulvestrant, 1.5 mg/kg/day); testosterone supplementation (durateston, 250 mg/kg, weekly); aromatase inhibitor (anastrozole, 0.2 mg /kg/day); testosterone plus anastrozole. Cotton ligatures were kept for 13 days, when pharmacological treatment was initiated. On day 14, the ligatures were removed. The rats were euthanized on the 17th or the 28th day (n = 5/group/period) for the evaluation of markers related to inflammation and bone. The tissue and serum samples were evaluated using a multiplexed immunoassay for the inflammatory targets. Radiographic and histologic analyses were performed to assess changes in tissues. Results: Blockage of androgen receptors had little effect on inflammatory cell count, although it tended to increase interleukin (IL)-4, vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) as well as decrease IL-1, tumor necrosis factor (TNF)-alpha, and IL-6. Flutamide also significantly impaired bone repair (P < 0.05) and had greater osteoclast count, although this last difference was not statistically significant. Testosterone supplementation significantly increased the inflammatory cell count, decreased the levels of IL-4, IL-10, IL-1 beta, IL-6, and TNF-alpha; and increased VEGF and EGF. Conclusion: The blockage of androgen receptors significantly impair bone repair in females through mechanisms that are different from those related to estrogen receptors. (AU)

Processo FAPESP: 14/02280-3 - Impacto de andrógenos na expressão de citocinas inflamatórias e marcadores de reparo tecidual sistemicamente e nos tecidos periodontais de ratas em condições de saúde, doença e reparo periodontal.
Beneficiário:Daniel Olivio Ribeiro
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 13/07833-8 - Participação de andrógenos na regulação da resposta imunoinflamatória e metabolismo ósseo associados à saúde, doença e reparo periodontal em fêmeas
Beneficiário:João Paulo Steffens
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/23116-4 - Impacto de andrógenos na resposta inflamatória, osteoclastogênese e expressão de receptores para hormônios sexuais nos tecidos periodontais de ratas em condições de saúde, doença e reparo periodontal
Beneficiário:Maria Carolina da Costa Albaricci
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 13/12014-6 - Participação de andrógenos na regulação da resposta imunoinflamatória e metabolismo ósseo associados à saúde, doença e reparo periodontal em fêmeas
Beneficiário:Luis Carlos Spolidorio
Linha de fomento: Auxílio à Pesquisa - Regular