Effects of high-impact exercise on the physical properties of bones of ovariectomized rats fed to a high-protein diet

Autor(es)

Shimano, R. C. ^[1] ; Yanagihara, G. R. ^[1] ; Macedo, A. P. ^[1] ; Yamanaka, J. S. ^[1] ; Shimano, A. C. ^[1] ; Tavares, J. M. R. S. ^[2] ; Issa, J. P. M. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biomecan Med & Reabil Aparelho, Sao Paulo - Brazil

^[2] Univ Porto, Fac Engn, Dept Engn Mecan, Inst Ciencia & Inovacao Engn Mecan & Engn Ind, Porto - Portugal

^[3] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Morfol Fisiol & Patol Basica, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS; v. 28, n. 5, p. 1523-1531, MAY 2018.
• Citações Web of Science: 1

Resumo

The aim of this study was to evaluate the effects of high-impact physical exercise as a prophylactic and therapeutic means in osteopenic bones of rats submitted to ovariectomy and protein diet intake. A total of 64 Wistar rats were divided into eight groups (n=8 each), being: OVX, ovx, standard diet and sedentary; OVXE, ovx, standard diet and jump; OVXP, ovx, high-protein diet and sedentary; and OVXEP, ovx, high-protein diet and jump; SH, sham, standard diet and sedentary; SHE, sham, standard diet and jump; SHP, sham, high-protein diet and sedentary; and SHEP, sham, high-protein diet and jump. OVX surgery consists of ovariectomy, and sham was the control surgery. The jumping protocol consisted of 20 jumps/day, 5days/week. The bone structure was evaluated by densitometry, mechanical tests, histomorphometric, and immunohistochemical analyses. A high-protein diet resulted in increased bone mineral density (P=.049), but decreased maximal load (P=.026) and bone volume fraction (P=.023). The benefits of physical exercise were demonstrated by higher values of the maximal load in the trained groups compared to the sedentary groups (P<.001). The sham groups had decreased immunostaining of osteocalcin (P=.004) and osteopontin (P=.010) compared to ovx groups. However, the high-protein diet (P=.005) and jump exercise (P=.017) resulted in lower immunostaining of osteopontin compared to the standard diet and sedentary groups, respectively. In this experimental model, it was concluded that ovariectomy and a high-fat diet can negatively affect bone tissue and the high-impact exercise was not enough to suppress the deleterious effects caused by the protein diet and ovariectomy. (AU)

• Processo FAPESP: 15/16194-4 - Papel da MMP-9 na patogênese da osteoporose em modelos de animais ateroscleróticos
• Beneficiário: João Paulo Mardegan Issa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/10733-8 - Análise tecidual e biomecânica de ossos de ratas alimentadas com ração hiperlipídica: estudo experimental da relação entre a massa gorda e a perda óssea
• Beneficiário: Antônio Carlos Shimano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/25879-8 - Análise tecidual e biomecânica de ossos de ratas ovariectomizadas alimentadas com ração hiperlipídica
• Beneficiário: Gabriela Rezende Yanagihara
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/26142-1 - Processamento e análise de Imagens obtidas por Microscopia Eletrônica de Varredura de ossos de ratas obesas e eutróficas
• Beneficiário: Gabriela Rezende Yanagihara
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado