Reduced methicillin-resistant Staphylococcus aureus biofilm formation in bone cavities by photodynamic therapy

Autor(es)

Dantas Araujo, Thalita Santos ^[1] ; Fernandes Rodrigues, Paolla Layanna ^[1] ; Santos, Mariana Sousa ^[1] ; de Oliveira, Janeide Muritiba ^[1] ; Rosa, Luciano Pereira ^{[2, 1]} ; Bagnato, Vanderlei Salvador ^[2] ; Blanco, Kate Cristina ^[2] ; da Silva, Francine Cristina ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Bahia, Multidisciplinary Hlth Inst, Vitoria Da Conquista, BA - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 21, p. 219-223, MAR 2018.
• Citações Web of Science: 5

Resumo

Photodynamic Therapy (PDT) is a promising alternative for the treatment of infectious bone lesions in the oral cavity. The objective of this study was to evaluate the antimicrobial effectiveness of PDT using blue LED associated with curcumin in methicillin-resistant Staphylococcus aureus biofilms (MRSA) in bovine bone cavities by fluorescence spectroscopy. Standardized suspensions of MRSA culture were inoculated into bone lesions to form biofilm. Forty bone species were distributed in three distinct groups: L-C-(control); L + C-(LED for 5 min); L-C + (curcumin incubation for 5 min) and L + C+ (PDT). Aliquots of 100 mu L were collected from the bone cavities after the treatments and were cultived in BHI for 24 h at 36 degrees C +/- 1 and bacterial colonies counting were performed. Statistical analysis were performed using the paired t-test and analysis of variance (ANOVA) for the variables studied. Results: The control and PDT groups presented statistically significant differences (p < 0.001). It was possible to reduce 3.666 1og10 CFU/mL of MRSA and a reduction in the fluorescence emitted after the treatments was observed. The MRSA reduction in biofilms by PDT was the most efficient treatmnent. There was a significant reduction of biofilms in the L + C-and non-PDT groups by fluorescence spectroscopy images. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs