Roles of auxin and ethylene in aerenchyma formation in sugarcane roots

Autor(es)

Tavares, E. Q. P. ^[1] ; Grandis, A. ^[1] ; Lembke, C. G. ^[2] ; Souza, G. M. ^[2] ; Purgatto, E. ^[3] ; De Souza, A. P. ^[1] ; Buckeridge, M. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Farmaceut, Dept Alimentos & Nutr Expt, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT SIGNALING & BEHAVIOR; v. 13, n. 3 2018.
• Citações Web of Science: 4

Resumo

Although the cross-talk between auxin and ethylene has been described during plant development, the role played by auxin upon gene expression during aerenchyma formation is poorly understood. Root aerenchyma formation results from the opening of gas spaces in the cortex. It is part of a developmental program (constitutive) or due to ethylene treatment or abiotic stress (induced) such as flooding and nutrient starvation. This process relies on programmed cell death and cell wall modifications. Here we followed development of aerenchyma formation in sugarcane along 5 cm from the root apex. As a constitutive process, the aerenchyma formation was observed in the cortex from the 3rd cm onwards. This occurred despite 1-methylcyclepropene (1-MCP) treatment, an inhibitor of ethylene perception. However, this process occurred while ethylene (and auxin) levels decreased. Within the aerenchyma formation zone, the concentration of ethylene is lower in comparison to the concentration in maize. Besides, the ratio between both hormones (ethylene and auxin) was around 1:1. These pieces of evidence suggest that ethylene sensitivity and ethylene-auxin balance may play a role in the formation of aerenchyma. Furthermore, the transcriptional analysis showed that genes related to cell expansion are up-regulated due to 1-MCP treatment. Our results help explaining the regulation of the formation constitutive aerenchyma in sugarcane. (AU)

• Processo FAPESP: 09/52840-7 - Centro de Processos Biológicos e Industriais para Biocombustíveis - CeProBIO
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 13/16225-1 - Interações DNA-proteína regulando a degradação de parede celular no desenvolvimento de aerênquima em raízes de cana de açúcar
• Beneficiário: Eveline Queiroz de Pinho Tavares
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/07586-5 - Investigação de mecanismos de regulação da degradação da parede celular em raízes de cana-de-açúcar durante a formação de aerênquima
• Beneficiário: Eveline Queiroz de Pinho Tavares
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/17104-5 - Degradação da parede celular em raízes de cana-de-açúcar
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático