Effects of Soy Lecithin Extender on Dog Sperm Cryopreservation

Autor(es)

Dalmazzo, Andressa ^[1] ; Losano, Joao Diego A. ^[1] ; Rocha, Carolina C. ^[1] ; Tsunoda, Roberta H. ^[1] ; Ramos Angrimani, Daniel de Souza ^[1] ; Mendes, Camilla M. ^[1] ; D'Avila Assumpcao, Mayra Elena O. ^[1] ; Nichi, Marcilio ^[1] ; Barnabe, Valquiria H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anim Reprod, Coll Vet Med & Anim Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANIMAL BIOTECHNOLOGY; v. 29, n. 3, p. 174-182, 2018.
• Citações Web of Science: 2

Resumo

Semen cryopreservation is an essential biotechnology in canine reproduction and during the cryopreservation process commonly egg yolk are used. The discrepancy in the egg yolk composition and the potential risk of disease dissemination are obstacles for semen exportation and use. Therefore, studies aiming to substitute egg yolk are extremely important. In this context, soy lecithin contains a low-density lipoprotein fraction, is an interesting alternative. Thus, the objective of this study was to compare extenders based on soy lecithin (several concentrations and forms) with egg yolk during the cryopreservation process of dog sperm. For this purpose, we used twelve dogs. Semen was evaluated at different time points (after refrigeration, glycerolization, and thawing), by motility analysis (CASA) and functional tests (e.g., membrane integrityeosin/nigrosin, acrosome integrityfast green/Bengal rose, mitochondrial activity3'3 diaminobenzidine, Chromatin susceptibility to acid-induced denaturationSCSA, and susceptibility to oxidative stressthiobarbituric acid reactive substances). The results indicated that egg yolk and lower concentrations of lecithin had similar effects on mitochondrial activity and motility. Thus, soy lecithin is a potentially viable alternative to egg yolk for the cryopreservation of dog semen. (AU)

• Processo FAPESP: 11/09222-0 - Utilização de lecitina de soja para a refrigeração e criopreservação do sêmen de cães
• Beneficiário: Renato Campanarut Barnabe
• Modalidade de apoio: Auxílio à Pesquisa - Regular