The combined and individual impact of diabetes and smoking on key subgingival periodontal pathogens in patients with chronic periodontitis

Autor(es)

Joaquim, C. R. ^[1] ; Miranda, T. S. ^[1] ; Marins, L. M. ^[1] ; Silva, H. D. P. ^[1] ; Feres, M. ^[1] ; Figueiredo, L. C. ^[1] ; Duarte, P. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Guarulhos, Dent Res Div, Dept Periodontol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PERIODONTAL RESEARCH; v. 53, n. 3, p. 315-323, JUN 2018.
• Citações Web of Science: 2

Resumo

Background and Objective: Comprehension of the similarities and differences in the composition of the subgingival microbiota of patients with diabetes mellitus (DM), smokers or smokers with DM is an important step in developing therapies specific for these groups at risk for periodontitis. Therefore, the aim of this study was to compare the combined and individual effects of DM and smoking on the levels and prevalence of key subgingival periodontal pathogens in patients with chronic periodontitis. Material and Methods: One hundred patients with generalized chronic periodontitis were allocated into one of the following groups: DM (n = 25, non-smokers with type 2 DM); S (n = 25, non-diabetic smokers); SDM (n = 25, smokers with type 2 DM); and control (n = 25, non-diabetic non-smokers). Two subgingival biofilm samples from healthy sites (probing depth and clinical attachment level 3mm and no bleeding) and 2 from diseased sites (probing depth and clinical attachment level 5mm and bleeding on probing) were analyzed by quantitative polymerase chain reaction for Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Eubacterium nodatum, Parvimonas micra, Fusobacterium nucleatum ssp. and Prevotella intermedia. Results: There were no differences among groups in the mean counts of the bacterial species studied, considering all sampled sites (healthy plus diseased sites). There were also no differences among groups regarding the prevalence of any bacteria species in healthy and diseased sites (P > .05). The mean P.micra count was significantly higher in the healthy sites of both smoking groups, than in those of the control group (P < .05). Conclusion: The subgingival levels and prevalence of the bacterial species studied are not significantly different in subjects with chronic periodontitis presenting DM, smokers or smokers with DM. In addition, DM and smoking, jointly and individually, do not considerably affect the subgingival levels of target periodontal pathogens in patients with chronic periodontitis. (AU)

• Processo FAPESP: 13/10354-4 - Influência dos fatores de risco para periodontite crônica no perfil imunoinflamatório sistêmico e periodontal
• Beneficiário: Poliana Mendes Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/23743-9 - Envolvimento dos antagonistas da sinalização Wnt/beta-catenina na periodontite crônica associada a fatores de risco
• Beneficiário: Tamires Szeremeske de Miranda
• Modalidade de apoio: Bolsas no Brasil - Doutorado