Photobiomodulation changes type 1 collagen gene expression by pulp fibroblasts

Autor(es)

Ribeiro Vitor, Luciana Lourenco ^[1] ; Oliveira Prado, Mariel Tavares ^[1] ; Lourenco Neto, Natalino ^[1] ; de Oliveira, Rodrigo Cardoso ^[2] ; Santos, Carlos Ferreira ^[2] ; Andrade Moreira Machado, Maria Aparecida ^[1] ; Oliveira, Thais Marchini ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Laser Physics; v. 28, n. 6 JUN 2018.
• Citações Web of Science: 0

Resumo

This study aimed to evaluate type 1 collagen (COL1) gene expression by human pulp fibroblasts from primary teeth (HPF) after the variation of photobiomodulation (PBM) parameters. HPF were obtained from a biorepository, used at 4th passage, and irradiated (InGaAlP-660 nm) varying the power and application time according to the following groups: G1: 1.2 J cm(-2)-05 mW-10 s; G2: 2.5 J cm(-2)-05 mW-20 s; G3: 3.7 J cm(-2)-05 mW-30 s; G4: 5.0 J cm(-2)-05 mW-40 s; G5: 6.2 J cm(-2)-05 mW-50 s; G6: 2.5 J cm(-2)-10 mW-10 s; G7: 3.7 J cm(-2)-15 mW-10 s; G8: 5.0 J cm(-2)-20 mW-10 s; G9: 6.2 J cm(-2)-25 mW-10 s. The control group (G10) was not irradiated and maintained with DMEM + 10% SFB. RT-PCR was used to evaluate COL1 gene expression at 6, 12, and 24 h after irradiation. Intra-and intergroup comparisons were performed by two-way ANOVA followed by Tukey test (p < 0.05). The intragroup comparison showed statistically significant differences among periods (p < 0.05). In G1, G9, and G10 higher COL1 gene expression occurred at 12 h (p = 0.07), while in G2 to G8, the peak of higher expression occurred at 6 h (p = 0.02). The intergroup comparison did not show statistically significant differences (p > 0.05). The energy densities from 2.5 to 5 J cm(-2), regardless of the variation in PBM parameters, biomodulated the COL1 gene expression. At the energy density of 6.2 J cm(-2), longer application time and smaller power changed the pattern of COL1 gene expression by pulp fibroblasts from human primary teeth. (AU)

• Processo FAPESP: 13/18886-5 - Influência de diferentes densidades de energia do laser de baixa intensidade em células derivadas da polpa de dentes decíduos
• Beneficiário: Thais Marchini de Oliveira Valarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular