Calcium chloride-enriched calcium aluminate cement promotes invitro osteogenesis

Autor(es)

Castro-Raucci, L. M. S. ^{[1, 2]} ; Teixeira, L. N. ^{[3, 1]} ; Barbosa, A. F. S. ^[2] ; Fernandes, R. R. ^[1] ; Raucci-Neto, W. ^[2] ; Jacobovitz, M. ^[4] ; Oliveira, I. R. ^[5] ; de Oliveira, P. T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Ribeirao Preto - Brazil

^[2] Univ Ribeirao Preto, Sch Dent, Ribeirao Preto - Brazil

^[3] Sao Leopoldo Mandic Inst & Res Ctr, Dept Oral Pathol, Campinas, SP - Brazil

^[4] Ctr Univ Paulista Cent, Sch Dent, Sao Carlos, SP - Brazil

^[5] Univ Vale Paraiba, Inst Res & Dev, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 51, n. 6, p. 674-683, JUN 2018.
• Citações Web of Science: 1

Resumo

AimTo evaluate the effects of 2.8% or 10% calcium chloride (CaCl2) in calcium aluminate cement (CAC) with either bismuth oxide (Bi2O3) or zinc oxide (ZnO) as radiopacifiers on the progression of osteogenic cell cultures. MethodologyRat calvaria-derived cells were grown on Thermanox((R)) coverslips for 24h and exposed to samples of (i) CACb: with 2.8% CaCl2 and 25% Bi2O3; (ii) CACb+: with 10% CaCl2 and 25% Bi2O3; (iii) CACz: with 2.8% CaCl2 and 25% ZnO; or (iv) CACz+: with 10% CaCl2 and 25% ZnO, placed on inserts. Nonexposed cultures served as the control. Calcium and phosphorus contents in culture media were quantified. The effects of the cementson cell apoptosis, cell viability and acquisition of the osteogenic cell phenotype were evaluated. Data werecompared by Kruskal-Wallis test (=5%). ResultsCACb+ promoted the highest levels of calcium in the culture media; CACz+, the lowest levels of phosphorus (P<0.05). CACz+ and CACb increased cell apoptosis (P<0.05). CACb reduced cell viability (P<0.05) and the expression of the osteoblastic phenotype. CACz+ and CACb+ promoted greater cell differentiation and matrix mineralization compared to CACz and CACb (P<0.05). ConclusionFor CAC with the lower CaCl2 content, the use of Bi2O3 was detrimental for osteoblastic cell survival and differentiation compared to ZnO, while CAC with the higher CaCl2 content supported the acquisition of the osteogenic cell phenotype invitro regardless of the radiopacifier used. Thus, CAC with 10% CaCl2 would potentially promote bone repair in the context of endodontic therapies. (AU)

• Processo FAPESP: 10/50936-4 - Efeitos de diferentes preparações de cimento de aluminato de cálcio sobre culturas de células osteogênicas e de células indiferenciadas da polpa dental
• Beneficiário: Paulo Tambasco de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/53310-1 - Efeitos de diferentes preparacoes de cimento de aluminato de calcio sobre culturas de celulas osteogenicas e de celulas indiferenciadas da polpa dental.
• Beneficiário: Larissa Moreira Spinola de Castro Raucci
• Modalidade de apoio: Bolsas no Brasil - Doutorado