Loci under selection and markers associated with host plant and host-related strains shape the genetic structure of Brazilian populations of Spodoptera frugiperda (Lepidoptera, Noctuidae)

Autor(es)

Silva-Brandao, Karina Lucas ^{[1, 2]} ; Peruchi, Aline ^[2] ; Seraphim, Noemy ^[3] ; Murad, Natalia Faraj ^[4] ; Carvalho, Renato Assis ^[5] ; Farias, Juliano Ricardo ^[6] ; Omoto, Celso ^[7] ; Consoli, Fernando Luis ^[7] ; Figueira, Antonio ^[2] ; Brandao, Marcelo Mendes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil

^[2] Univ Sao Paulo, Ctr Energia Nucl Agr, Lab Melhoramento Plantas, Campus Luiz de Queiroz, Piracicaba, SP - Brazil

^[3] Inst Fed Educ Ciencia & Tecnol Sao Paulo, Campus Campinas CTI Renato Archer, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Programa Posgrad Genet & Biol Mol, Campinas, SP - Brazil

^[5] Monsanto Brasil Ltda, Sao Paulo, SP - Brazil

^[6] Inst Phytus, Dept Entomol, Rua Duque de Caxias, Santa Maria, RS - Brazil

^[7] Univ Sao Paulo, Dept Entomol & Acarol, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 5 MAY 22 2018.
• Citações Web of Science: 0

Resumo

We applied the ddRAD genotyping-by-sequencing technique to investigate the genetic distinctiveness of Brazilian populations of the noctuid moth Spodoptera frugiperda, the fall armyworm (FAW), and the role of host-plant association as a source of genetic diversification. By strain-genotyping all field-collected individuals we found that populations collected from corn were composed primarily of corn-strain individuals, while the population collected from rice was composed almost entirely of rice-strain individuals. Outlier analyses indicated 1,184 loci putatively under selection (ca. 15% of the total) related to 194 different Gene Ontologies (GOs); the most numerous GOs were nucleotide binding, ATP binding, metal ion binding and nucleic-acid binding. The association analyses indicated 326 loci associated with the host plant, and 216 loci associated with the individual strain, including functions related to Bacillus thuringiensis and insecticide resistance. The genetic-structure analyses indicated a moderate level of differentiation among all populations, and lower genetic structure among populations collected exclusively from corn, which suggests that the population collected from rice has a strong influence on the overall genetic structure. Populations of S. frugiperda are structured partially due to the host plant, and pairs of populations using the same host plant are more genetically similar than pairs using different hosts. Loci putatively under selection are the main factors responsible for the genetic structure of these populations, which indicates that adaptive selection on important traits, including the response to control tactics, is acting in the genetic differentiation of FAW populations in Brazil. (AU)

• Processo FAPESP: 11/00417-3 - Biologia de sistemas aplicada a agricultura: análise de transcriptomas e interactomas
• Beneficiário: Marcelo Mendes Brandao
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/16266-7 - Genômica populacional: uma nova abordagem para estudos de especiação em insetos devido ao uso de hospedeiros aplicada ao desenvolvimento de estratégias sustentáveis de MIP
• Beneficiário: Karina Lucas da Silva-Brandão
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores