Genomic identification of microbial species adhering to maxillofacial prostheses and susceptibility to different hygiene protocols

Autor(es)

Pinheiro, Juliana Barchelli ^[1] ; Vomero, Marina Peris ^[1] ; do Nascimento, Cassio ^[1] ; Watanabe, Evandro ^[2] ; Oliveira Paranhos, Helena de Freitas ^[1] ; Coto, Neide Pena ^[3] ; Dias, Reinaldo Brito ^[3] ; de Oliveira, Viviane Cassia ^[1] ; Silva-Lovato, Claudia Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Dent Mat & Prostheses, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Restorat Dent, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Dent Sao Paulo, Dept Maxillofacial Surg Prostheses & Traumatol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 34, n. 1, p. 15-25, 2018.
• Citações Web of Science: 1

Resumo

This study investigated the microbial colonization of maxillofacial prostheses and support tissues using the Checkerboard DNA-DNA hybridization method, and the efficacy of 0.12% chlorhexidine gluconate, 10% Ricinus communis solutions, or brushing, on colony forming unit (CFU) reduction in monospecies biofilms (Candida glabrata, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Enterococcus faecalis, and Pseudomonas aeruginosa) formed on two silicones (MDX 4-4210 and Bio-Skin). Biofilm was harvested from 43 maxillofacial prosthesis wearers for detection of 38 species of microorganisms. The CFU counts of the six above mentioned species were recorded after using the hygiene protocols. All 38 investigated species were identified in prostheses and tissues, with a higher prevalence in the prostheses. 0.12% chlorhexidine gluconate immersion showed the greatest antimicrobial effectiveness, followed by mechanical brushing protocols. MDX 4-4210 silicone produced lower CFU counts than Bio-Skin. (AU)

• Processo FAPESP: 12/24853-0 - Identificação da microbiota de próteses faciais e avaliação laboratorial da efetividade de diferentes soluções de higiene.
• Beneficiário: Juliana Barchelli Pinheiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado