Structural and functional stabilization of bacteriophage particles within the aqueous core of a W/O/W multiple emulsion: A potential biotherapeutic system for the inhalational treatment of bacterial pneumonia

Autor(es)

Rios, Alessandra C. ^[1] ; Vila, Marta M. D. C. ^[1] ; Lima, Renata ^[1] ; Del Fiol, Fernando S. ^[1] ; Tubino, Matthieu ^[2] ; Teixeira, Jose A. ^[3] ; Balcao, Victor M. ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sorocaba, Intelligent Biosensing & Biomol Stabilizat Res Gr, Lab Biofilms & Bacteriophages UNISO, PhageLab, Sorocaba, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil

^[3] Univ Minho, CEB Ctr Biol Engn, Braga - Portugal

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 64, p. 177-192, JAN 2018.
• Citações Web of Science: 4

Resumo

The increase of antibiotic-resistant bacteria is growing every day, most likely associated with the indiscriminate use of these antimicrobials or even with evolutionary adaptability of bacteria to their environment. This situation brings a need to develop new alternatives to conventional antibiotics, and thus the application of strictly lytic bacteriophages has been proposed as an alternative (or complement) to the former, allowing release of the natural predators of bacteria directly where they are needed the most: the infection site. The main advantages of bacteriophages to treat infections is the maintenance of a high concentration of bacteriophage particles in the action site while any viable target bacteria still exist, coupled to the production of enzymes that hydrolyze the polymeric matrix of bacterial biofilms promoting penetration and antibacterial action. In the research effort entertained herein, the potential for protection and stabilization of strictly lyric bacteriophages with broad spectrum capable of infecting Pseudomonas aeruginosa, so as to maintain their structure and functionality, was investigated via encapsulation within the aqueous-core of lipid nanodroplets integrating a W/O/W multiple emulsion system, aiming at developing isotonic derivative solutions thereof for administration by nebulization. (AU)

• Processo FAPESP: 16/08884-3 - PneumoPhageColor - desenvolvimento de um kit de biodetecção colorimétrica de Pseudomonas aeruginosa com base em partículas fágicas
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/12234-4 - TransAppIL - permeação cutânea de entidades proteicas estabilizadas estruturalmente e funcionalmente, carreadas por líquidos iônicos
• Beneficiário: Marta Maria Duarte Carvalho Vila
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/03181-6 - PneumoPhageKill: desenvolvimento de sistema terapêutico contendo bacteriófagos nanoencapsulados líticos para Pseudomonas aeruginosa, para tratamento de pneumonia bacteriana por aerossolização
• Beneficiário: Marta Maria Duarte Carvalho Vila
• Linha de fomento: Auxílio à Pesquisa - Regular