| Texto completo | |
| Autor(es): |
Guissoni Campos, Leila M.
[1, 2]
;
Hataka, Alessandre
[3]
;
Vieira, Isis Z.
[1]
;
Buchaim, Rogerio L.
[4, 2]
;
Robalinho, Isadora F.
[2]
;
Arantes, Giovanna E. P. S.
[2]
;
Viegas, Joyce S.
[2]
;
Bosso, Henrique
[2]
;
Bravos, Rafael M.
[2]
;
Pinato, Luciana
[1]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ UNESP, Marilia - Brazil
[2] Univ Marilia UNIMAR, Marilia - Brazil
[3] Sao Paulo State Univ UNESP, Botucatu, SP - Brazil
[4] Univ Sao Paulo, Bauru - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN PHYSIOLOGY; v. 9, FEB 9 2018. |
| Citações Web of Science: | 3 |
| Resumo | |
Oscillations of brain proteins in circadian rhythms are important for determining several cellular and physiological processes in anticipation of daily and seasonal environmental rhythms. In addition to the suprachiasmatic nucleus, the primary central oscillator, the cerebellum shows oscillations in gene and protein expression. The variety of local circuit rhythms that the cerebellar cortex contains influences functions such as motivational processes, regulation of feeding, food anticipation, language, and working memory. The molecular basis of the cerebellar oscillator has been demonstrated by ``clock gene{''} expression within cells of the cerebellar layers. Genetic and epidemiological evidence suggests that disruption of circadian rhythms in humans can lead to many pathological conditions. Despite this importance, data about clock gene and protein expression in the cerebellum of diurnal (day-active) species, specifically primates, is currently poorly explored, mainly in regard to cellular identity, as well as the relationship with other molecules also involved in cerebellar functions. These studies could contribute to clarification of the possiblemechanisms behind cerebellar rhythmicity. Considering that calcium binding proteins (CaBPs) play crucial roles in preserving and modulating cerebellar functions and that clock gene expression can be controlled by afferent projections or paracrine circadian signals such as the hormone melatonin, the present study aimed to describe cellular identities, distribution patterns and day/night expression changes in PER1, PER2, CaBPs, and MT1 and MT2 melatonin receptors in the cerebellar cortex of a diurnal primate using conventional fluorescence and peroxidase- antiperoxidase immunocytochemical techniques. PER1 and PER2 immunoreactive (IR) cells were observed in the Purkinje cells of the cerebellum, and MT1 and MT2 receptors were localized around Purkinje cells in the Pj layer in Bergmann cells. This identity was confirmed by the S100 beta-IR of these cells. The highest expression of PER seen in the daytime analysis coincided with the highest expression of melatonin receptors. CaBPs showed day/nightmorphological and density changes in the cerebellar cortex. The presence of the same temporal variations in the expression of PER in the Pj neurons and in MT1 and MT2 receptors in Bergmann cells indicates a possible relation between these cells during the rhythmic processing of the cerebellum, in addition to the CaBP temporal morphological and density changes. (AU) | |
| Processo FAPESP: | 18/00563-9 - Proteínas clock circadianas e receptores de melatonina em neuronios e células da glia no cerebelo do sapajus apella |
| Beneficiário: | Luciana Pinato |
| Modalidade de apoio: | Auxílio à Pesquisa - Publicações científicas - Artigo |
| Processo FAPESP: | 11/51495-4 - Produção de melatonina por células do sistema nervoso central em condições de injúria |
| Beneficiário: | Luciana Pinato |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |