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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Improved solution for vitrification of Prochilodus lineatus embryos based on the reduction in risk factors: Toxicity, osmotic responses and ice-nucleation

Texto completo
Autor(es):
Costa, Raphael da Silva [1] ; Silva Souza, Fabricio Marcal [1] ; Senhorini, Jose Augusto [2] ; Ribeiro, Douglas de Castro [1] ; Bashiyo-Silva, Cristiane [1] ; Zacheo Coelho, Geovanna Carla [1] ; Verissimo-Silveira, Rosicleire [1] ; Ninhaus-Silveira, Alexandre [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Sao Paulo - Brazil
[2] Natl Ctr Res & Conservat Continental Fish, Chico Mendes Inst Biodivers Conservat, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: AQUACULTURE RESEARCH; v. 49, n. 2, p. 793-800, FEB 2018.
Citações Web of Science: 2
Resumo

The objective of our work was to describe a low toxicity cryoprotectant solution that allowed vitreous solid formation. Embryos of Prochilodus lineatus were submitted to sensitivity evaluations of six internal cryoprotectants (dimethyl sulphoxide - Me2SO, dimethyl acetamide - DMA, dimethyl formamide - DMF, methanol - MET, glycerol - GLY and 1,2-propanediol - PROP) at concentrations of 1-6 M; and two external cryoprotectants (sucrose - SUC and glucose - GLU) at concentrations of 0.1-1 M for 20 min. The capacity of the cryoprotectant solutions to exchange heat with the medium and to form glassy solids was evaluated by immersing 10 mu l of cryoprotectant in liquid nitrogen. The PROP had a high survival rate at all concentrations evaluated, and was the only substance that allowed a vitreous solid formation. Thus, it is concluded that the PROP-6 M was the most adequate solution for embryonic vitrification processes, because heat exchange between the system (PROP 6 M/embryos/liquid nitrogen) was faster than for other cryoprotectants and combinations thereof; has low toxicity, promote high rates of dehydration in short periods, and reach the vitreous state, being a good candidate to be used in the tests of embryonic vitrification. (AU)

Processo FAPESP: 13/02588-5 - CRIOPRESERVAÇÃO DE EMBRIÕES DE Prochilodus lineatus SUBMETIDOS À ELETROPORAÇÃO E AO ULTRASSOM.
Beneficiário:Raphael da Silva Costa
Modalidade de apoio: Bolsas no Brasil - Doutorado