Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling

Autor(es):Mostrar menos -

Ge, Zengxiang ^[1] ; Bergonci, Tabata ^{[2, 3]} ; Zhao, Yuling ^[1] ; Zou, Yanjiao ^[3] ; Du, Shuo ^[1] ; Liu, Ming-Che ^[3] ; Luo, Xingju ^[1] ; Ruan, Hao ^[4] ; Garcia-Valencia, Liliana E. ^{[3, 5]} ; Zhong, Sheng ^[1] ; Hou, Saiying ^[1] ; Huang, Qingpei ^[1] ; Lai, Luhua ^[4] ; Moura, Daniel S. ^[2] ; Gu, Hongya ^{[1, 6]} ; Dong, Juan ^[7] ; Wu, Hen-Ming ^[3] ; Dresselhaus, Thomas ^[8] ; Xiao, Junyu ^[1] ; Cheung, Alice Y. ^[3] ; Qu, Li-Jia ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Peking Univ, Coll Life Sci, Peking Tsinghua Ctr Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871 - Peoples R China

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, BR-13418900 Piracicaba, SP - Brazil

^[3] Univ Massachusetts, Dept Biochem & Mol Biol, Mol & Cell Biol Program, Plant Biol Program, Amherst, MA 01003 - USA

^[4] Peking Univ, Coll Chem & Mol Engn, Beijing 100871 - Peoples R China

^[5] Univ Nacl Autonoma Mexico, Fac Quim, Dept Bioquim, Mexico City 04510, DF - Mexico

^[6] Natl Plant Gene Res Ctr Beijing, Beijing 100101 - Peoples R China

^[7] Rutgers State Univ, Waksman Inst Microbiol, Piscataway, NJ 08854 - USA

^[8] Univ Regensburg, Biochem Zentrum Regensburg, Cell Biol & Plant Biochem, D-93053 Regensburg - Germany

• Tipo de documento: Artigo Científico
• Fonte: Science; v. 358, n. 6370, p. 1596-1599, DEC 22 2017.
• Citações Web of Science: 67

Resumo

In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and the female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at the pollen tube surface. We found that two such receptors in Arabidopsis, BUPS1 and BUPS2, and their peptide ligands, RALF4 and RALF19, are pollen tube-expressed and are required to maintain pollen tube integrity. BUPS1 and BUPS2 interact with receptors ANXUR1 and ANXUR2 via their ectodomains, and both sets of receptors bind RALF4 and RALF19. These receptor-ligand interactions are in competition with the female-derived ligand RALF34, which induces pollen tube bursting at nanomolar concentrations. We propose that RALF34 replaces RALF4 and RALF19 at the interface of pollen tube-female gametophyte contact, thereby deregulating BUPS-ANXUR signaling and in turn leading to pollen tube rupture and sperm release. (AU)

• Processo FAPESP: 14/14487-1 - Mecanismos de percepção do peptídeo AtRALF1: seus receptores e a dissociação entre o fluxo iônico e a regulação negativa da expansão celular
• Beneficiário: Daniel Scherer de Moura
• Modalidade de apoio: Auxílio à Pesquisa - Regular