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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Differences in the Aspect Ratio of Gold Nanorods that Induce Defects in Cell Membrane Models

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Autor(es):
Lins, Paula M. P. [1] ; Marangoni, Valeria S. [1] ; Uehara, Thiers M. [1] ; Miranda, Paulo B. [1] ; Zucolotto, Valtencir [1] ; Cancino-Bernardi, Juliana [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Nanomed & Nanotoxicol Grp, Phys Inst Sao Carlos, CP 369, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Langmuir; v. 33, n. 50, p. 14286-14294, DEC 19 2017.
Citações Web of Science: 4
Resumo

Understanding the interactions between biomolecules and nanomaterials is of great importance for many areas of nanomedicine and bioapplications. Although studies in this area have been performed, the interactions between cell membranes and nanoparticles are not fully understood. Here, we investigate the interactions that occur between the Langmuir monolayers of dipalmitoylphosphatidyl glycerol (DPPG) and dipalmitoylphosphatidyl choline (DPPC) with gold nanorods (NR) with three aspect ratios and gold nanoparticles. Our results showed that the aspect ratio of the NRs influenced the interactions with both monolayers, which suggest that the physical morphology and electrostatic forces govern the interactions in the DPPG NR system, whereas the van der Waals interactions are predominant in the DPPC NR systems. Size influences the expansion isotherms in both systems, but the lipid tails remain conformationally ordered upon expansion, which suggests phase separation between the lipids and nanomaterials at the interface. The coexistence of lipid and NP regions affects the elasticity of the monolayer. When there is coexistence between two phases, the elasticity does not reflect the lipid packaging state but depends on the elasticity of the NP islands. Therefore, the results corroborate that nanomaterials influence the packing and the phase behavior of the mimetic cell membranes. For this reason, developing a methodology to understand the membrane nanomaterial interactions is of great importance. (AU)

Processo FAPESP: 12/03570-0 - Nanomateriais aplicados em nanomedicina: desenvolvimento de modelos de membrana e estudos in vitro para avaliação da toxicidade
Beneficiário:Juliana Cancino Bernardi
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 12/15630-7 - Isolamento de membranas de linhagem celular para aplicação em modelos de membrana em estudos de nanotoxicidade
Beneficiário:Paula Maria Pincela Lins
Linha de fomento: Bolsas no Brasil - Iniciação Científica