Differences in the Aspect Ratio of Gold Nanorods that Induce Defects in Cell Membrane Models

Autor(es)

Lins, Paula M. P. ^[1] ; Marangoni, Valeria S. ^[1] ; Uehara, Thiers M. ^[1] ; Miranda, Paulo B. ^[1] ; Zucolotto, Valtencir ^[1] ; Cancino-Bernardi, Juliana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Nanomed & Nanotoxicol Grp, Phys Inst Sao Carlos, CP 369, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Langmuir; v. 33, n. 50, p. 14286-14294, DEC 19 2017.
• Citações Web of Science: 7

Resumo

Understanding the interactions between biomolecules and nanomaterials is of great importance for many areas of nanomedicine and bioapplications. Although studies in this area have been performed, the interactions between cell membranes and nanoparticles are not fully understood. Here, we investigate the interactions that occur between the Langmuir monolayers of dipalmitoylphosphatidyl glycerol (DPPG) and dipalmitoylphosphatidyl choline (DPPC) with gold nanorods (NR) with three aspect ratios and gold nanoparticles. Our results showed that the aspect ratio of the NRs influenced the interactions with both monolayers, which suggest that the physical morphology and electrostatic forces govern the interactions in the DPPG NR system, whereas the van der Waals interactions are predominant in the DPPC NR systems. Size influences the expansion isotherms in both systems, but the lipid tails remain conformationally ordered upon expansion, which suggests phase separation between the lipids and nanomaterials at the interface. The coexistence of lipid and NP regions affects the elasticity of the monolayer. When there is coexistence between two phases, the elasticity does not reflect the lipid packaging state but depends on the elasticity of the NP islands. Therefore, the results corroborate that nanomaterials influence the packing and the phase behavior of the mimetic cell membranes. For this reason, developing a methodology to understand the membrane nanomaterial interactions is of great importance. (AU)

• Processo FAPESP: 12/03570-0 - Nanomateriais aplicados em nanomedicina: desenvolvimento de modelos de membrana e estudos in vitro para avaliação da toxicidade
• Beneficiário: Juliana Cancino Bernardi
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/15630-7 - Isolamento de membranas de linhagem celular para aplicação em modelos de membrana em estudos de nanotoxicidade
• Beneficiário: Paula Maria Pincela Lins
• Linha de fomento: Bolsas no Brasil - Iniciação Científica