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First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups

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Autor(es):
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Carvalho, Pedro C. [1] ; de Oliveira, Ezequiel A. [1, 2] ; Bertollo, Luiz A. C. [1] ; Yano, Cassia F. [1] ; Oliveira, Claudio [3] ; Decru, Eva [4] ; Jegede, Oladele I. [5] ; Hatanaka, Terumi [1] ; Liehr, Thomas [6] ; Al-Rikabi, Ahmed B. H. [6] ; Cioffi, Marcelo de B. [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[2] Estado Educ Mato Grosso Seduc MT, Cuiaba - Brazil
[3] Univ Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP - Brazil
[4] Royal Museum Cent Africa, Sect Vertebrates Ichthyol, Tervuren - Belgium
[5] Adamawa State Univ, Dept Fisheries & Aquaculture, Mubi - Nigeria
[6] Univ Hosp Jena, Inst Human Genet, Jena - Germany
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN GENETICS; v. 8, DEC 12 2017.
Citações Web of Science: 6
Resumo

Hepsetidae is a small fish family with only the genus Hepsetus, with six described species distributed throughout the South, Central and Western regions of Africa, showing a close relationship with the Alestidae and some Neotropical fish families. However, no cytogenetic information is available for both Hepsetidae and Alestidae species, thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing new cytogenetic data for Hepsetus odoe, including the standard karyotype, C-banding, repetitive DNAs mapping, comparative genomic hybridization (CGH) and whole chromosome painting (WCP), providing chromosomal patterns and subsidies for comparative cytogenetics with other characiform families. Both males and females H. odoe have 2n = 58 chromosomes (10m + 28sm + 20st/a), with most of the C-band positive heterochromatin localized in the centromeric and subtelomeric regions. Only one pair of chromosomes bears proximal 5S rDNA sites in the short arms, contrasting with the 18S rDNA sequences which are located in the terminal regions of four chromosome pairs. Clear interstitial hybridization signals are evidenced for the U1 and U2 snDNA probes, but in only one and two chromosome pairs, respectively. Microsatellite motifs are widely distributed in the karyotype, with exception for the (CGG)(10), (GAA)(10) and (GAG)(10) probes, which highlight conspicuous interstitial signals on an unique pair of chromosomes. Comparative data from conventional and molecular cytogenetics, including CGH and WCP experiments, indicate that H. odoe and some Erythrinidae species, particularly Erythrinus erythrinus, share similar chromosomal sequences suggesting some relatedness among them, although bearing genomic specificities in view of their divergent evolutionary histories. (AU)

Processo FAPESP: 16/17556-0 - Inter-relações evolutivas da família Erythrinidae (Teleostei, Characiformes). IV: citogenética comparativa na família Hepsetidae
Beneficiário:Pedro Cristovão Carvalho
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 17/08471-3 - Hibridização genômica comparativa, microdissecção e pintura cromossômica em Hepsetus akawo (Characiformes, Hepsetidae)
Beneficiário:Pedro Cristovão Carvalho
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Iniciação Científica
Processo FAPESP: 16/21411-7 - Evolução cariotípica e de cromossomos sexuais em peixes neotropicais. Parte II
Beneficiário:Luiz Antonio Carlos Bertollo
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/26508-3 - Filogenia da hiperdiversa ordem Characiformes (Teleostei: Ostariophysi) utilizando elementos ultraconservados
Beneficiário:Claudio de Oliveira
Linha de fomento: Auxílio à Pesquisa - Temático