| Texto completo | |
| Autor(es): |
Morozesk, Mariana
[1]
;
Franqui, Lidiane S.
[2, 3]
;
Mansano, Adrislaine S.
[4]
;
Martinez, Diego Stefani T.
[2, 3]
;
Fernandes, Marisa N.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Fed Univ Sao Carlos UFSCar, Physiol Sci Dept, Washington Luiz Hwy, Km 235, BR-13565905 Sao Carlos, SP - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, Polo Alta Tecnol Campinas 2, Giuseppe Maximo Scolfaro St 10-000, BR-13083970 Campinas, SP - Brazil
[3] Univ Campinas UNICAMP, Sch Technol, Paschoal Marino St 1888, BR-13484332 Limeira, SP - Brazil
[4] Fed Univ Sao Carlos UFSCar, Dept Ecol & Evolutionary Biol, Washington Luiz Hwy, Km 235, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | AQUATIC TOXICOLOGY; v. 200, p. 136-147, JUL 2018. |
| Citações Web of Science: | 5 |
| Resumo | |
The widespread production and application of carbon nanotubes (CNT) have raising concerns about their release into the environment and, the joint toxicity of CNT with pre-existing contaminants needs to be assessed. This is the first study that investigated the co-exposure of oxidized multiwalled carbon nanotubes (ox-MWCNT) and cadmium (Cd) using a zebrafish liver cell line (ZFL). Two in vitro co-exposure protocols differing by the order of ox-MWCNT interaction with Cd and fetal bovine serum (FBS) proteins were evaluated. Ox-MWCNT was physical and chemical characterized and its adsorption capacity and colloidal stability in cell culture medium was determined in both protocols. Cytotoxicity was investigated by M'FT, neutral red, trypan blue, lactate dehydrogenase assays and the necrosis and apoptosis events were determined using flow cytometer. The Cd presence in medium did not interfere in the protein corona composition of MWCNT but the order of interaction of FBS and Cd interfered in its colloidal stability and metal adsorption rate. The ox-MWCNT increased Cd toxicity at low concentration probably by a ``Trojan horse{''} and/or synergistic effect, and induced apoptosis and necrosis in ZFL cells. Although it was not observed differences of toxicity between protocols, the interaction of ox-MWCNT first with Cd led to its precipitation in cell culture medium and, as a consequence, to a possible false viability result by neutral red assay. Taken together, it was evident that the order of compounds interactions disturbs the colloidal stability and affects the in vitro toxicological assays. Considering that Protocol A showed more ox-MWCNT stability after interaction with Cd, this protocol is recommended to be adopted in future studies. (AU) | |
| Processo FAPESP: | 14/05701-0 - Respostas integradas de curimbatá, Prochilodus lineatus (Teleostei) às nanopartículas de dióxido de titânio (TiO2) e identificação de biomarcadores para o monitoramento ambiental |
| Beneficiário: | Marisa Narciso Fernandes |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 14/50906-9 - INCT 2014: em Materiais Complexos Funcionais (INOMAT) |
| Beneficiário: | Fernando Galembeck |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |