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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Biological response to self-etch adhesive after partial caries removal in rats

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Autor(es):
Da Silva, Adriana Fernandes [1] ; Marques, Marcelo Rocha [2] ; De Oliveira Da Rosa, Wellington Luiz [1] ; Chaves Tarquinio, Sandra Beatriz [3] ; Rosalen, Pedro Luiz [4] ; Barros, Silvana Pereira [5]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Fed Univ Pelotas UFPel, Sch Dent, Dept Restorat Dent, Pelotas - Brazil
[2] State Univ Campinas UNICAMP, Sch Dent, Dept Morphol, Piracicaba - Brazil
[3] Fed Univ Pelotas UFPel, Sch Dent, Dept Semiol & Clin, Pelotas - Brazil
[4] State Univ Campinas UNICAMP, Sch Dent, Dept Physiol Sci, Piracicaba - Brazil
[5] Univ N Carolina, Ctr Oral & Syst Dis, Dept Periodontol, Sch Dent, Chapel Hill, NC 27515 - USA
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: CLINICAL ORAL INVESTIGATIONS; v. 22, n. 6, p. 2161-2173, JUL 2018.
Citações Web of Science: 2
Resumo

The purposes of this study were to evaluate a model of slow caries progression and to investigate the performance of a self-etch adhesive system for partial caries removal. Rat molars were infected with Streptococcus sobrinus 6715 culture. Different time points were analyzed: days 78, 85, and 95 (+/- 2). After this, the samples were processed for morphological analysis. Additionally, the first molars were restored with zinc oxide and eugenol (IRM (TM); Dentsply; Brazil) or adhesive system (Clearfil SE Bond (TM); Kuraray Medical; Japan) 78 days after caries induction. After, 3 or 15 days post-treatment, the animals were euthanized, and their mandibles were processed for morphological analysis, classified by means of scores, and submitted to statistical analysis. Subsequently, immunohistochemical analysis was performed for osteonectin (OSN) and transforming growth factor-1 (TGF-1) expression. According to the caries induction model used, on day 95 greater inflammatory infiltration (p < 0.001), and more extensive degradation of secondary/primary dentin were demonstrated than on day 78 (p < 0.05). Furthermore, the restorative materials presented similar performance (p > 0.05) and proved to be fundamental to control the carious lesion. The TGF-1 and OSN were shown to be active during the caries process. The slow caries lesion model was feasible for morphological analysis of the dentin-pulp complex. The self-etch adhesive system triggered no acute inflammatory infiltration or pulp necrosis, instead it seemed to stimulate early pulp repair. Clearfil SE Bond (TM) applied directly on caries-affected dentin did not predispose to pulp inflammation; instead, it appeared to provide early biological benefits. (AU)

Processo FAPESP: 04/00276-7 - Analise imuno-histoquimica de componentes da matriz extracelular em resposta a aplicacao de diferentes tratamentos apos remocao de carie em dentina profunda, estudo em ratos.
Beneficiário:Silvana Pereira Barros
Modalidade de apoio: Auxílio à Pesquisa - Regular