Photobiomodulation therapy and vitamin C on longevity of cell sheets of human dental pulp stem cells

Autor(es)

Pedroni, Ana C. F. ^[1] ; Diniz, Ivana M. A. ^{[1, 2]} ; Abe, Gabriela L. ^[1] ; Moreira, Maria S. ^[3] ; Sipert, Carla R. ^[1] ; Marques, Marcia M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo - Brazil

^[2] Univ Fed Minas Gerais, Sch Dent, Dept Restorat Dent, Belo Horizonte, MG - Brazil

^[3] Univ Ibirapuera, Sch Dent, Post Grad Program, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Physiology; v. 233, n. 10, p. 7026-7035, OCT 2018.
• Citações Web of Science: 4

Resumo

Photobiomodulation therapy (PBMT) can improve processes relevant to tissue regeneration, such as survival, proliferation, migration, and differentiation of cells, including stem cells. Thus, PBMT could be applied as auxiliary therapy for tissue regeneration. Cell sheets (CSs) induced by vitamin C (VC) can generate large amount of cells, which would also be useful for tissue regeneration. VC and PBMT cause opposite effects on cell metabolism (e.g., VC is antioxidative, and PBMT generates reactive oxygen species); however, hDPSC CSs were formed when VC and PBMT+VC were applied. Thus, this study showed that PBMT does not interfere with the formation of cell sheets induced by VC. Additionally, PBMT improved the functional differentiation of the cells isolated from the CSs. Thus, due to the clinical benefits of PBMT, the association of this therapy with cell sheets seems promising for future applications in tissue regeneration. (AU)

• Processo FAPESP: 17/16777-5 - Investigação do efeito de cell sheet de células-tronco da polpa dentária humana associada ou não à biofotônica (terapia de fotobiomodulação e/ou à terapia fotodinâmica antimicrobiana) na regeneração tecidual em murinos
• Beneficiário: Marcia Martins Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular