Fatty acid influence on Prochilodus lineatus (Characiformes, Prochilodontidae) embryo cryopreservation parameters.

Autor(es)

Costa, Raphael da Silva ^[1] ; Silva Souza, Fabricio Marcal ^[2] ; Senhorini, Jose Augusto ^[3] ; Bashiyo-Silva, Cristiane ^[2] ; Verissimo-Silveira, Rosicleire ^[2] ; Zacheo Coelho, Geovanna Carla ^[2] ; Siqueira-Silva, Diogenes Henrique ^[4] ; Ribeiro, Cristiele da Silva ^[2] ; Ninhaus-Silveira, Alexandre ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci Humanities & Exact Sci, Sao Jose Do Rio Preto - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Nat Sci & Engn, Dept Biol & Anim Sci, Ilha Solteira - Brazil

^[3] Chico Mendes Inst Biodivers Conservat CEPTA ICMBi, Natl Ctr Res & Conservat Continental Fish, Pirassununga - Brazil

^[4] Univ Fed Sul Sudeste Para, Inst Estudos Saude Biol IESB, Maraba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AQUACULTURE RESEARCH; v. 49, n. 8, p. 2714-2722, AUG 2018.
• Citações Web of Science: 0

Resumo

This study aimed to evaluate the vitellogenic transference and incorporation of long-chain polyunsaturated fatty acids (LC-PUFA) into the membranes of Prochilodus lineatus embryos, aiming to increase the permeability to cryoprotectants and resistance to electric fields. One hundred thirty broodstock of P.lineatus were fed with control (C) or fish oil-supplemented diets (FO) for 12months. The fatty acid (FA) profle was determined using gas chromatography. For the neutral fraction, the FO group had a decrease in monounsaturated fatty acids (MUFA) and an increase in n3PUFA and, n6PUFA. To test for cryoprotectant toxicity, embryos were exposed for 20min to a cryoprotectant solution of 1,2-Propanediol (Prop) at a concentration of 5 or 6 molar (M). For FO, a reduction in survival of 33.1% was observed in 5M, and no survival was observed at 6M. Embryo samples were exposed the six polarized electric fields (3.4-51.6 joules), and with 11.2J of energy, the control group exhibited reduced survival in 98.3% of the fish, whereas the FO presented superior resistance, exhibiting a survival similar to that of the OJ up to 40.2J. We conclude that FA were transferred between P.lineatus broodstock to the embryos, with an increase in LC-PUFA resulting in lower survival rates in the cryoprotectant test in the FO group and a greater physical plasticity of FO embryos to electrical field tests. (AU)

• Processo FAPESP: 15/10115-5 - Vitrificação de embriões de Astyanax altiparanae (Garutti & Britski, 2000)
• Beneficiário: Alexandre Ninhaus Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/02588-5 - CRIOPRESERVAÇÃO DE EMBRIÕES DE Prochilodus lineatus SUBMETIDOS À ELETROPORAÇÃO E AO ULTRASSOM.
• Beneficiário: Raphael da Silva Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado