Methylene blue internalization and photodynamic action against clinical and ATCC Pseudomonas aeruginosa and Staphyloccocus aureus strains

Autor(es)

Correia Pereira, Andre Henrique ^[1] ; Pinto, Juliana Guerra ^[1] ; Alves Freitas, Mirian Aparecida ^[1] ; Fontana, Leticia Correa ^[1] ; Soares, Cristina Pacheco ^[2] ; Ferreira-Strixino, Juliana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, IP&D, Lab Terapia Fotodinam, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba, IP&D, Lab Dinam Compartimentos Celulares, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 22, p. 43-50, JUN 2018.
• Citações Web of Science: 2

Resumo

Bacterial infections have been a major challenge to health. Increasing resistance to antimicrobial agents, according to World Health Organization, could be the major cause of death until 2050. Photodynamic therapy emerges as an alternative in microbial inactivation, due to its selectivity and to decreasing or dismissing antibiotic use. This study aimed at evaluating, in vitro, the internalization of the Methylene Blue and its photo dynamic activity against a clinical and ATCC strain of Pseudomonas aeruginosa and Staphyloccocus aureus. Thus, the strains were incubated with MB in concentrations of 100, 300 e 500 mu g/ml and then irradiated with a LED (+/- 660 nm) at fluence of 10 and 25 J/cm(2). The MB internalization was evaluated using a confocal microscope (Zeiss LSM 700), to capture the MB and the DAPI (for DNA staining). It was possible to observe that the MB was internalized by the bacterial cells, in all concentrations tested. The CFU/ml count demonstrated significant reduction (p <= 0,01) at the average 5.0 logs comparing with control group for the two species in all the tested concentrations. In conclusion, the strains tested were capable of internalizing the MB. PDT with MB was able to decrease the growth of the tested strains in vitro, being a promising alternative to the future treatment of infections caused by these species. (AU)

• Processo FAPESP: 13/12284-3 - Avaliação da ação da terapia fotodinâmica em Leishmania sp.: estudo in vitro
• Beneficiário: Juliana Ferreira Strixino
• Modalidade de apoio: Auxílio à Pesquisa - Regular