DNA sequence database as a tool to identify decapod crustaceans on the Sao Paulo coastline

Autor(es)

Mantelatto, Fernando L. ^[1] ; Terossi, Mariana ^[1] ; Negri, Mariana ^[1] ; Buranelli, Raquel C. ^[1] ; Robles, Rafael ^{[1, 2]} ; Magalhaes, Tatiana ^[1] ; Tamburus, Ana Francisca ^[1] ; Rossi, Natalia ^[1] ; Miyazaki, Mayara J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Lab Bioecol & Crustacean Syst, Ribeirao Preto, SP - Brazil

^[2] Univ Autonoma Campeche, Fac Ciencias Quim Biol, San Francisco De Campech, Campeche - Mexico

• Tipo de documento: Artigo Científico
• Fonte: MITOCHONDRIAL DNA PART A; v. 29, n. 5, p. 805-815, 2018.
• Citações Web of Science: 13

Resumo

DNA barcoding has emerged as an efficient tool for taxonomy and other biodiversity fields. The vast and speciose group of decapod crustaceans is not an exception in the current scenario and comparing short DNA fragments has enabled researchers to overcome some taxonomic impediments to help broadening knowledge on the diversity of this group of crustaceans. Brazil is considered as an important area in terms of global marine biodiversity and some regions stand out in terms of decapod fauna, such as the Sao Paulo coastline. Thus, the aim of this study is to obtain sequences of the mitochondrial markers (COI and 16S) for decapod crustaceans distributed at the Sao Paulo coastline and to test the accuracy of these markers for species identification from this region by comparing our sequences to those already present in the GenBank database. We sampled along almost the 300 km of the Sao Paulo coastline from estuaries to offshore islands during the development of a multidisciplinary research project that took place for 5 years. All the species were processed to obtain the DNA sequences. The diversity of the decapod fauna on the Sao Paulo coastline comprises at least 404 species. We were able to collect 256 of those species and sequence of at least one of the target genes from 221. By testing the accuracy of these two DNA markers as a tool for identification, we were able to check our own identifications, including new records in GenBank, spot potential mistakes in GenBank, and detect potential new species. (AU)

• Processo FAPESP: 10/50188-8 - Crustáceos decápodes: multidisciplinaridade na caracterização da biodiversidade marinha do estado de São Paulo (taxonomia, espermiotaxonomia, biologia molecular e dinâmica populacional) (Biodiversidade Marinha)
• Beneficiário: Fernando Luis Medina Mantelatto
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático