Radiolabeled GX1 Peptide for Tumor Angiogenesis Imaging

Autor(es)

de Oliveira, Erica Aparecida ^{[1, 2]} ; Faintuch, Bluma Linkowski ^[1] ; Seo, Daniele ^[1] ; Barbezan, Angelica Bueno ^[3] ; Funari, Ana ^[3] ; Targino, Roselaine Campos ^[4] ; Moro, Ana Maria ^[4]

Afiliação do(s) autor(es)

^[1] Inst Energy & Nucl Res, Radiopharm Ctr, Ave Prof Lineu Prestes 2242, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci, Ave Prof Lineu Prestes, 580 Bloco 17, BR-05508900 Sao Paulo, SP - Brazil

^[3] Inst Energy & Nucl Res, Radiat Technol Ctr, Ave Prof Lineu Prestes 2242, BR-05508000 Sao Paulo - Brazil

^[4] Butantan Inst, Lab Biopharmacol Anim Cells, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 185, n. 4, p. 863-874, AUG 2018.
• Citações Web of Science: 0

Resumo

Early and accurate detection of primary or metastatic tumors is of great value in staging, treatment management, and prognosis. Tumor angiogenesis plays an essential role in the growth, invasion, and metastatic spread of solid cancers, and so, is a promising approach for tumor imaging. The GX1 (CGNSNPKSC) peptide was identified by phage display library and has been investigated as a marker for human cancers. This study aims to evaluate the Tc-99m-HYNIC-PEG(4)-c (GX1) as a biomarker for tumor imaging. Our results showed that GX1 specifically binds to tumor cells in vitro. SKMEL28 and MDA-MB231 cells achieved total binding peak at 60 min of incubation. For B16F10 and MKN45 cells, the total and specific binding were similar during all time points, while A549 cell line showed rapid cellular total uptake of the tracer at 30 min of incubation. Biodistribution showed low non-specific uptakes and rapid renal excretion. Melanoma tumors showed enhanced GX1 uptake in animal model at 60 min, and it was significantly blocked by cold peptide. The radiotracer showed tumor specificity, especially in melanomas that are highly vascularized tumors. In this sense, it should be considered in future studies, aiming to evaluate degree of angiogenesis, progression, and invasion of tumors. (AU)

• Processo FAPESP: 11/12405-0 - Desenvolvimento de radiotraçadores angiogênicos para diagnóstico de glioma: estudo em modelo animal
• Beneficiário: Érica Aparecida de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado