Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato

Autor(es)

Quintero, Fabiola OCampo ^[1] ; Pinto, Layra G. ^[1] ; Barsalobres-Cavallari, Carla F. ^[1] ; Campos Arcuri, Mariana de Lara ^[1] ; Pino, Lilian Ellen ^[2] ; Pereira Peres, Lazaro Eustaquio ^[2] ; Maluf, Mirian P. ^[3] ; Maia, Ivan G. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Genet, BR-18618689 Botucatu, SP - Brazil

^[2] Univ Sao Paulo, Dept Biol Sci LCB, ESALQ, BR-13418900 Piracicaba, SP - Brazil

^[3] Agron Inst Campinas, Embrapa Coffee & Coffee Ctr Alcides Carvalho, BR-13012970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Plant Cell Reports; v. 37, n. 9, p. 1257-1268, SEP 2018.
• Citações Web of Science: 0

Resumo

A seed maturation protein gene (CaSMP) from Coffea arabica is expressed in the endosperm of yellow/green fruits. The CaSMP promoter drives reporter expression in the seeds of immature tomato fruits. In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5'-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (similar to 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool. (AU)

• Processo FAPESP: 07/59963-1 - Amplificacao, clonagem e sequenciamento de promotores de cafe (coffea arabica) especificos de fruto e ubiquos.
• Beneficiário: Layra Gabriella Pinto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/03241-1 - Caracterização dos genes da família das lacases de eucalipto (Eucalyptus grandis)
• Beneficiário: Mariana de Lara Campos Arcuri
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/54361-9 - Desenvolvimento de ferramentas para a expressao genica em cafe (coffea arabica): caracterizacao de promotores tecido-especificos e ubiquos.
• Beneficiário: Ivan de Godoy Maia
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50746-3 - Análise funcional dos promotores dos genes SP, 14-3-3 e cagpdh de café (Coffea arabica)
• Beneficiário: Fabiola Ocampo Quintero
• Modalidade de apoio: Bolsas no Brasil - Doutorado