Study of the chromatographic parameters of ultra-high-performance supercritical fluid chromatography and method development using a design of experiments approach for the quantification of pesticides in lettuce

Autor(es)

de Lima, Karen Priscila ^[1] ; Sales Fontes Jardim, Isabel Cristina ^[1] ; Breitkreitz, Marcia Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas UNICAMP, Inst Quim, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SEPARATION SCIENCE; v. 41, n. 16 AUG 2018.
• Citações Web of Science: 0

Resumo

We examine the potential of ultra-high-performance supercritical fluid chromatography for multiresidue quantification of ten pesticides commonly applied to lettuce and compares it to ultra-high-performance liquid chromatography. Initially, a thorough study of the stationary and mobile phase composition and injection solvent was carried out. In a second step, a chemometric approach based on design of experiments was used to simultaneously study the influence of temperature, pressure, and percentage of ethanol on the retention, resolution and symmetry of the peaks. Using this approach, it was possible to obtain the Design Space, a robust region where complete separation of the analytes was achieved, with acceptable peak shape. Both methods were validated according to the figures of merit: selectivity, linearity, quantification limit, accuracy (in terms of recovery), and precision (repeatability and intermediate precision) and used to quantify the pesticides in lettuce samples. Comparing both techniques, it was concluded that the limits of quantification, accuracy, and precision were similar. However, in supercritical fluid chromatography, a reduced volume of organic solvent was used, the method was faster and generated lower amounts of residues. (AU)

• Processo FAPESP: 16/05636-9 - Desenvolvimento de métodos analíticos inovadores para analitos de interesse farmacêutico empregando cromatografia com fluído supercrítico de ultra alta eficiência (UHPSFC) em associação com ferramentas quimiométricas
• Beneficiário: Márcia Cristina Breitkreitz
• Modalidade de apoio: Auxílio à Pesquisa - Regular