| Texto completo | |
| Autor(es): |
de Lima Lira, Aline Aparecida
[1]
;
de-Oliveira, Marilia Garcia
[1]
;
Sabo Inoue, Amanda Harumi
[1]
;
Beltrame, Giovanna Rossi
[1]
;
da Silva Duarte, Alberto Jose
[1, 2]
;
Victor, Jefferson Russo
[3, 1, 2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Med Sch, Div Clin Dermatol, Lab Med Invest LIM 56, Sao Paulo - Brazil
[2] Univ Sao Paulo, Med Sch, Div Pathol, Sao Paulo - Brazil
[3] Laureate Int Univ, Div Environm Hlth, FMU, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ALLERGOLOGIA ET IMMUNOPATHOLOGIA; v. 46, n. 5, p. 454-459, SEP-OCT 2018. |
| Citações Web of Science: | 5 |
| Resumo | |
Background: IL-17-producing B cells can be identified in both mice and human and were named B17 cells. The role of B17 cells still needs to be elucidated and its inflammatory or regulatory functions remain controversial. Objective: We evaluate the effect of maternal immunization with OVA on offspring B cells that produces IL-17 and can show a regulatory potential by IL-10 production. Methods: C57BL/6 WT, IL-10(-/-) or CD28(-/-) female mice were immunized or not with OVA in Alum, and immunized females were boosted after 10 and 20 days. Immunized and non immunized females were mated, and pups from both groups were evaluated at 3 or 20 days old (d.o.). Some offspring from the aforementioned two groups were immunized with OVA at 3 d.o., boosted after 10 days and evaluated at 20 d.o. Results: Maternal immunization with OVA induced offspring B cells to produce IL-17 at higher intensity compared to the control group of offspring at 3 d.o. This effect was maintained until 20 d.o. and even after neonatal immunization with OVA. The co-production of IL-10 on offspring IL-17 + B cells is up-regulated in response to maternal immunization with OVA. Maternal immunization with OVA on IL-10(-/-)mice reveals reduced percentage and mean of fluorescence intensity of IL-17 on B cells of offspring. Conclusion: Preconception OVA immunization can induce offspring B cells that produce IL-17 at higher intensity and co-produce mainly IL-10. This could be the reason why B17 cells had been described in the literature with controversial roles upon their regulatory function. (C) 2018 SEICAP. Published by Elsevier Espana, S.L.U. All rights reserved. (AU) | |
| Processo FAPESP: | 13/22820-0 - "Influência da imunização materna com Ovalbumina na maturação tímica de linfócitos TCD4+, TCD8+, nTreg, Tgd e B da prole" |
| Beneficiário: | Marília Garcia de Oliveira |
| Linha de fomento: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 17/18558-9 - Avaliação in vitro da influência do repertório IgG murino sobre a maturação de linfócitos B e Tgamma-delta com potencial regulador e/ou modulador |
| Beneficiário: | Amanda Harumi Sabô Inoue |
| Linha de fomento: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 15/17256-3 - "Investigação dos mecanismos de inibição da resposta de hipersensibilidade tipo I da prole mediada pela imunização materna murina: modulação de citocinas, indução de linfócitos T e B reguladores e influência da natureza do alérgeno." |
| Beneficiário: | Alberto José da Silva Duarte |
| Linha de fomento: | Auxílio à Pesquisa - Regular |