Preconceptional allergen immunization can induce offspring IL-17 secreting B cells (B17): do they share similarities with regulatory B10 cells?

Author(s)

de Lima Lira, Aline Aparecida ^[1] ; de-Oliveira, Marilia Garcia ^[1] ; Sabo Inoue, Amanda Harumi ^[1] ; Beltrame, Giovanna Rossi ^[1] ; da Silva Duarte, Alberto Jose ^{[1, 2]} ; Victor, Jefferson Russo ^{[3, 1, 2]}

Affiliation

^[1] Univ Sao Paulo, Med Sch, Div Clin Dermatol, Lab Med Invest LIM 56, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Med Sch, Div Pathol, Sao Paulo - Brazil

^[3] Laureate Int Univ, Div Environm Hlth, FMU, Sao Paulo - Brazil

• Document type: Journal article
• Source: ALLERGOLOGIA ET IMMUNOPATHOLOGIA; v. 46, n. 5, p. 454-459, SEP-OCT 2018.
• Web of Science Citations: 5

Abstract

Background: IL-17-producing B cells can be identified in both mice and human and were named B17 cells. The role of B17 cells still needs to be elucidated and its inflammatory or regulatory functions remain controversial. Objective: We evaluate the effect of maternal immunization with OVA on offspring B cells that produces IL-17 and can show a regulatory potential by IL-10 production. Methods: C57BL/6 WT, IL-10(-/-) or CD28(-/-) female mice were immunized or not with OVA in Alum, and immunized females were boosted after 10 and 20 days. Immunized and non immunized females were mated, and pups from both groups were evaluated at 3 or 20 days old (d.o.). Some offspring from the aforementioned two groups were immunized with OVA at 3 d.o., boosted after 10 days and evaluated at 20 d.o. Results: Maternal immunization with OVA induced offspring B cells to produce IL-17 at higher intensity compared to the control group of offspring at 3 d.o. This effect was maintained until 20 d.o. and even after neonatal immunization with OVA. The co-production of IL-10 on offspring IL-17 + B cells is up-regulated in response to maternal immunization with OVA. Maternal immunization with OVA on IL-10(-/-)mice reveals reduced percentage and mean of fluorescence intensity of IL-17 on B cells of offspring. Conclusion: Preconception OVA immunization can induce offspring B cells that produce IL-17 at higher intensity and co-produce mainly IL-10. This could be the reason why B17 cells had been described in the literature with controversial roles upon their regulatory function. (C) 2018 SEICAP. Published by Elsevier Espana, S.L.U. All rights reserved. (AU)

• FAPESP's process: 15/17256-3 - "Mechanisms involved on offspring allergy development regulation by maternal immunization: role of cytokines, induction of T and B regulatory cells and allergen nature."
• Grantee: Alberto José da Silva Duarte
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/22820-0 - Influence of maternal immunization with ovalbumin in thymic maturation of lymphocytes TCD4+, TCD8+, nTreg, T gamma delta and B on offspring
• Grantee: Marília Garcia de Oliveira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/18558-9 - Influence of murine IgG repertoire on the maturation of B and gamma-deltaT cells with modulatory and/or regulatory potential in vitro
• Grantee: Amanda Harumi Sabô Inoue
• Support Opportunities: Scholarships in Brazil - Scientific Initiation