Effect of maternal immunization with ovalbumin in mice in the activation of dendritic cells and in the generation of regulatory T cells of the offspring

• Grant number: 09/12051-3
• Support type: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2010
• Effective date (End): July 31, 2011
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal researcher: Maria Notomi Sato
• Grantee: Bruno Pacola Muniz
• Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The allergen sensitization of the immune system can have a very early beginning, even at the gestational period or in the first months of postnatal life. In the allergic response there is a skewed Th2 response, which together with the pronely Th2 shift response at the neonatal period, are factors that may contribute to the development of the allergic diseases early in life. Indeed, this fact becomes relevant the investigation of the strategies to control the development of the type I hypersensitivity response at very early phase of life, employing experimental models. Previously, it was demonstrated by our investigation group, that the maternal immunization with allergens, in mice, is able to prevent the development of the IgE response in offspring (Fusaro et al., 2002, 2007, 2009; Victor et al., 2003). Several mechanisms could be acting in the regulation of the offspring allergic response due to the mother immunization (vaccination). In this context, until now it was not determined the participation of the dendritic cells and their influence on the generation of regulatory T cells in the offspring mice. The aim of the Project is to evaluate the regulatory mechanism involved in the maternal immunization with ovalbumin (OVA) in the IgE response of offspring. Thus, the ex vivo dendritic cells (DCs) CD11c+CD11b+ maturation profile of offspring from immunized or non-immunized mothers, though the analysis of the costimulatory expression and the cytokines production as well as by their effect in the antigen-specific proliferative response and the generation of T CD4+CD25+FoxP3+ cells when co-cultured with cells from DO11.10 transgenic mice. Furthermore, the number of the T CD4+CD25+FoxP3+ cells and their ability to control antigen-specific proliferative responsiveness will be evaluated in the immunized mothers and immunized offspring at the neonatal period. The in vitro influence of the DC pulsing with the complex (IgG plus OVA) will be evaluate on the proliferative responsiveness, T CD4+ cell cycle, and generation of T CD4+CD25+FoxP3+ cells from DO11.10 mice.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MUNIZ, BRUNO PACOLA; VICTOR, JEFFERSON RUSSO; OLIVEIRA, LUANA DE MENDONCA; DE LIMA LIRA, ALINE APARECIDA; PERINI, ADENIR; OLIVO, CLARICE ROSA; ARANTES-COSTA, FERNANDA MAGALHAES; MARTINS, MILTON ARRUDA; DA SILVA DUARTE, ALBERTO JOSE; SATO, MARIA NOTOMI. Tolerogenic microenvironment in neonatal period induced by maternal immunization with ovalbumin. Immunobiology, v. 219, n. 5, p. 377-384, MAY 2014. Web of Science Citations: 10.