Influence of maternal immunization with ovalbumin in thymic maturation of lymphocytes TCD4+, TCD8+, nTreg, T gamma delta and B on offspring

• Grant number: 13/22820-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2014
• End date: December 31, 2014
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Jefferson Russo Victor
• Grantee: Marília Garcia de Oliveira
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In the last decade our research group has been studying the effects of maternal immunization with allergens such as dust mites Dermatophagoides pteronyssinus (Der p) and ovalbumin (OVA) in regulating the allergic response of the offspring of mice. This research currently focuses on the mechanisms mediated by the transfer of maternal antibodies and their effect on the splenic lymphocytes of offspring (Victor Muniz et al. 2010). However, as reviewed by our group (Rigato, Fusaro et al., 2009), the state can induce maternal immune interactions during early maturation of lymphocytes in the thymus. These interactions can be mediated by passive transfer of antibodies and/or maternal factors such as cytokines that can influence the clonal selection and/or cytokine production by lymphocyte maturation in the thymus. In the meantime, this project aims to initiate the investigation of the effect of immunization or maternal atopy on the maturation of lymphocytes intratímicos offspring. Therefore, we will evaluate the cytokine production by CD4, CD8, nTreg, Tgd and B matured in the thymus of offspring of mothers immunized with OVA or not in the neonatal period and after immunization. The results indicate that lymphocyte population has greater importance in the mechanism studied and this will be investigated as to their maturation stages. This proposal establishes an unprecedented assessment in clarifying the mechanisms related to the regulation of the allergic response of the offspring resulting from maternal immunization. This contributes to the improvement of future vaccination strategies maternal allergen to protect newborns.(AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, MARLIA GARCIA; OLIVEIRA, LUANA DE MENDONCA; DE LIMA LIRA, ALINE APARECIDA; SGNOTTO, FABIO DA RESSUREICAO; DA SILVA DUARTE, ALBERTO JOSE; SATO, MARIA NOTOMI; VICTOR, JEFFERSON RUSSO. Preconception allergen sensitization can induce B10 cells in offspring: a potential main role for maternal IgG. ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY, v. 13, APR 17 2017. (15/17256-3, 13/22820-0, 10/09004-0)

DE LIMA LIRA, ALINE APARECIDA; DE-OLIVEIRA, MARILIA GARCIA; SABO INOUE, AMANDA HARUMI; BELTRAME, GIOVANNA ROSSI; DA SILVA DUARTE, ALBERTO JOSE; VICTOR, JEFFERSON RUSSO. Preconceptional allergen immunization can induce offspring IL-17 secreting B cells (B17): do they share similarities with regulatory B10 cells?. ALLERGOLOGIA ET IMMUNOPATHOLOGIA, v. 46, n. 5, p. 454-459, SEP-OCT 2018. (15/17256-3, 13/22820-0, 17/18558-9)