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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dissecting the Subcellular Localization, Intracellular Trafficking, Interactions, Membrane Association, and Topology of Citrus Leprosis Virus C Proteins

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Autor(es):
Leastro, Mikhail Oliveira [1] ; Kitajima, Elliot Watanabe [2] ; Silva, Marilia Santos [3] ; Resende, Renato Oliveira [4] ; Freitas-Astua, Juliana [5, 1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Inst Biol, Dept Bioquim Fitopatol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Fitopatol & Nematol, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil
[3] Embrapa Recursos Genet & Biotecnol, Lab Bioimagem, Brasilia, DF - Brazil
[4] Univ Brasilia, Dept Biol Celular, Brasilia, DF - Brazil
[5] Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN PLANT SCIENCE; v. 9, SEP 11 2018.
Citações Web of Science: 1
Resumo

Citrus leprosis (CL) is a re-emergent viral disease affecting citrus crops in the Americas, and citrus leprosis virus C (CiLV-C), belonging to the genus Cilevirus, is the main pathogen responsible for the disease. Despite the economic importance of CL to the citrus industry, very little is known about the performance of viral proteins. Here, we present a robust in vivo study around functionality of p29, p15, p61, MP, and p24 CiLV-C proteins in the host cells. The intracellular sub-localization of all those viral proteins in plant cells are shown, and their co-localization with the endoplasmic reticulum (ER), Golgi complex (GC) ( p15, MP, p61 and p24), actin filaments (p29, p15 and p24), nucleus (p15), and plasmodesmata (MP) are described. Several features are disclosed, including i) ER remodeling and redistribution of GC apparatus, ii) trafficking of the p29 and MP along the ER network system, iii) self-interaction of the p29, p15, and p24 and hetero-association between p29-p15, p29-MP, p29-p24, and p15-MP proteins in vivo. We also showed that all proteins are associated with biological membranes; whilst p15 is peripherally associated, p29, p24, and MP are integrally bound to cell membranes. Furthermore, while p24 exposes an N-cytoplasm-C-lumen topology, p29, and p15 are oriented toward the cytoplasmic face of the biological membrane. Based on our findings, we discuss the possible performance of each protein in the context of infection and a hypothetical model encompassing the virus spread and sites for replication and particle assembly is suggested. (AU)

Processo FAPESP: 14/08458-9 - Vírus de plantas transmitidos por Brevipalpus (Acari: Tenuipalpidae) - VTB: levantamento, identificação, caracterização molecular, filogenia; relações vírus/vetor/hospedeira; biologia, taxonomia e manejo do vetor
Beneficiário:Elliot Watanabe Kitajima
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/10249-1 - Estudo dos processos infectivos do vírus da leprose do citros (CiLV-C), com ênfase na construção de clone infeccioso e caracterização das proteínas virais.
Beneficiário:Mikhail Oliveira Leastro
Linha de fomento: Bolsas no Brasil - Pós-Doutorado