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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Silencing of Apis mellifera dorsal genes reveals their role in expression of the antimicrobial peptide defensin-1

Texto completo
Autor(es):
Lourenco, A. P. [1, 2] ; Florecki, M. M. [1] ; Simoes, Z. L. P. [3] ; Evans, J. D. [4]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Sao Paulo - Brazil
[2] Univ Fed Vales Jequitinhonha & Mucuri, Fac Ciencias Biol & Saude, Dept Ciencias Biol, Campus JK, BR-39100000 Diamantina, MG - Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Sao Paulo - Brazil
[4] USDA ARS, Bee Res Lab, Beltsville, MD - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Insect Molecular Biology; v. 27, n. 5, p. 577-589, OCT 2018.
Citações Web of Science: 2
Resumo

Like all other insects, two key signalling pathways {[}Toll and immune deficiency (Imd)] regulate the induction of honey bee immune effectors that target microbial pathogens. Amongst these effectors are antimicrobial peptides (AMPs) that are presumed to be produced by the nuclear factors kappa B (NF-B) Dorsal and Relish from the Toll and Imd pathways, respectively. Using in silico analysis, we previously proposed that the honey bee AMP defensin-1 was regulated by the Toll pathway, whereas hymenoptaecin was regulated by Imd and abaecin by both the Toll and Imd pathways. Here we use an RNA interference (RNAi) assay to determine the role of Dorsal in regulating abaecin and defensin-1. Honey bees have two dorsal genes (dorsal-1 and dorsal-2) and two splicing isoforms of dorsal-1 (dorsal-1A and dorsal-1B). Accordingly, we used both single and multiple (double or triple) isoform knockdown strategies to clarify the roles of dorsal proteins and their isoforms. Down-regulation of defensin-1 was observed for dorsal-1A and dorsal-2 knockdowns, but abaecin expression was not affected by dorsal RNAi. We conclude that defensin-1 is regulated by Dorsal (Toll pathway). (AU)

Processo FAPESP: 03/07041-2 - Estudo dos genes codificadores dos peptídeos antimicrobianos e de outros genes efetores na resposta imune em Apis mellifera
Beneficiário:Anete Pedro Lourenço
Linha de fomento: Bolsas no Brasil - Doutorado