The reversal effect of physical exercise on aging-related increases in APPL2 content in skeletal muscle

Autor(es):Mostrar menos -

Canciglieri, Paulo Henrique ^[1] ; Kuga, Gabriel Keine ^[2] ; Munoz, Vitor Rosetto ^[1] ; Gaspar, Rafael Calais ^[1] ; da Rocha, Alisson Luiz ^[3] ; Breda, Leonardo ^[1] ; Anaruma, Chadi Pellegrini ^[2] ; Minuzzi, Luciele Guerra ^[1] ; Ramos da Silva, Adelino Sanchez ^[3] ; Cintra, Dennys Esper ^{[4, 5]} ; de Moura, Leandro Pereira ^{[6, 1, 2, 4]} ; Ropelle, Eduardo Rochete ^{[6, 1, 4]} ; Pauli, Jose Rodrigo ^{[6, 1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Postgrad Program Movement Sci, Rio Claro, SP - Brazil

^[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[4] Univ Estadual Campinas, OCRC, Lab Cell Signaling, Campinas, SP - Brazil

^[5] Univ Campinas UNICAMP, Sch Appl Sci, Lab Nutr Genom LabGeN, Limeira, SP - Brazil

^[6] Univ Campinas UNICAMP, Sch Appl Sci, CEPECE Ctr Res Sport Sci, Limeira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 210, p. 209-213, OCT 1 2018.
• Citações Web of Science: 0

Resumo

Aims: The aim of this study was to evaluate the effects of aging on intracellular adiponectin signaling and the possible therapeutic effect of physical exercise. Main methods: Fischer 344 rats were distributed in the following groups: Young (3 months old); Sedentary Old (Old, 27 months old); and Old Exercised (Old-Exe, 27 months old), which were subjected to a short-term exercise training protocol. Key findings: The results showed that the old rats presented glucose intolerance without increased adiposity. However, short-term exercise training reversed this disorder, which was associated with a decrease in the pleckstrin homology domain, phosphotyrosine-binding domain, and leucine zipper motif (APPL) isoform 2 (APPL2) content. The APPL isoform 1 (APPL1) and TRB3 (Tribbles homolog 3) contents were not altered. Akt phosphorylation was only increased in the old exercised rats. There was a reduction in the content of adiponectin receptor 1 in the old rats. Significance: The short-term exercise training protocol was able to decrease APPL2 content in the skeletal muscle, which was accompanied by an improvement in the glucose tolerance of the old Fischer 344 rats. These findings provide new evidence supporting the role of physical exercise as a non-pharmacological therapeutic intervention to attenuate age-related deficits. (AU)

• Processo FAPESP: 16/18488-8 - O papel do exercício físico nas vias moleculares de captação de glicose em camundongos nocautes para GLUT-4
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular