Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease

Autor(es)

Haro Chavez, Natali Lisette ^[1] ; de Avila, Erica Dorigatti ^[1] ; Barbugli, Paula Aboud ^[1] ; de Oliveira, Regiane Cristina ^[2] ; de Foggi, Camila Cristina ^[1] ; Longo, Elson ^[2] ; Vergani, Carlos Eduardo ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Fed Sao Carlos, CDMF, UFSCar, Washington Luis Km 235, POB 676, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 170, p. 505-513, OCT 1 2018.
• Citações Web of Science: 3

Resumo

Silver tungstate (alpha-Ag2WO4) microcrystals have shown encouraging results regarding their antimicrobial activity. However, in addition to the promising outcomes in fighting oral disease, cytotoxic tests are mandatory for screening new materials for biological applications. Here, we developed a better understanding of the effects of microcrystals on the behavior of both human gingival fibroblast (HGF) cells and three-dimensional (3D) collagen matrices. To perform these experiments, the lowest concentration of alpha-Ag2WO4 capable of preventing the visible growth of Candida albicans (C. albicans) planktonic cells was defined as the test concentration, and it ranged from 0.781 (C1) to 7.81 (C2) to 78.1 (C3) mu g/mL. Complete medium and lysis buffer (LB) served as negative (C-) and positive (C+) controls, respectively. The effect of the microcrystal concentration on the morphology, remodeling and proliferation of HGF cells was evaluated by different approaches. Quantitative and qualitative assessments demonstrated that alpha-Ag2WO4 did not affect the mitochondria] enzymatic activity of HGF cells cultured in a monolayer or the cell viability within 3D collagen matrices. These experiments showed that alpha-Ag2WO4 at the C2 concentration did not damage the genomic DNA. The development of new materials is attractive for the possible treatment of diseases and for avoiding indiscriminate prescribing of antibiotics. These findings provide information on the effect of alpha-Ag2WO4 on cell behavior and reveal that these microcrystals are non-cytotoxic against human gingival cells over a sufficient period to measure the hazard potential. (AU)

• Processo FAPESP: 15/25124-0 - Efeito das propriedades antimicrobianas e citotóxicas de superfícies de titânio revestidas com microcristais de ±-Ag2WO4 e -Ag2MoO4
• Beneficiário: Carlos Eduardo Vergani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
• Beneficiário: Elson Longo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 15/03654-7 - Ação antimicrobiana de óxidos bimetálicos e de revestimento depositado a plasma sobre resina acrílica
• Beneficiário: Camila Cristina de Foggi
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/03567-7 - Modificação das propriedades físico-químicas das superfícies de titânio para abutments de implantes com diferentes tipos de revestimentos e avaliação do efeito do tratamento no desenvolvimento de um complexo biofilme oral: um estudo preliminar in vitro
• Beneficiário: Érica Dorigatti de Avila
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado