Effects of Manufacturing and Finishing Techniques of Feldspathic Ceramics on Surface Topography, Biofilm Formation, and Cell Viability for Human Gingival Fibroblasts

Autor(es)

Contreras, L. P. C. ^[1] ; Dal Piva, A. M. O. ^[2] ; Ribeiro, F. C. ^[3] ; Anami, L. C. ^[4] ; Camargo, S. E. A. ^[3] ; Jorge, A. O. C. ^[3] ; Bottino, M. A. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Inst Sci & Technol, Dept Dent Mat & Prosthodont, Sao Jose Dos Campos - Brazil

^[2] Sao Paulo State Univ Unesp, Dept Dent Mat & Prosthodont, Sao Jose Dos Campos - Brazil

^[3] Sao Paulo State Univ Unesp, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos - Brazil

^[4] Univ Santo Amaro, Dept Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Operative Dentistry; v. 43, n. 6, p. 593-601, NOV-DEC 2018.
• Citações Web of Science: 2

Resumo

Purpose: Feldspathic ceramic restorations can be obtained by different techniques (stratification or computer-aided design/computer-aided manufacturing {[}CAD/CAM] blocks) and finishing procedures (polishing or glaze application). This study evaluated the effects of techniques and finishing procedures on surface properties, biofilm formation, and viability of human gingival fibroblasts (FMM-1) in contact with these materials. Methods and Materials: Ceramic specimens were obtained through a stratification technique (Vita VM9) and from CAD/CAM blocks (Vita Blocs Mark II; both Vita Zahnfabrik) and their surfaces were finished by polishing (ceramiste diamond rubbers + polishing paste; ``p{''} subgroups) or glaze spray application + sintering ({''}g{''} subgroups). Roughness (Ra and RSm parameters) and surface free energy (SFE) were measured. Early biofilm formation of Streptococcus mutans, Streptococcus sanguinis, and Candida albicans was evaluated by counting colony-forming units (CFU). MTT (3-{[}4.5-dimethyl-thiazol-2-y1-]-2.5-diphenyl tetra-zolium bromide) cytotoxicity test evaluated cellular viability for the growth of FMM-1 after 24 hours and seven days of contact. Scanning electron microscopy (SEM) and three-dimensional optical profilometry were performed to qualitatively analyze the surface. Data were analyzed by analysis of variance, Tukey test, and t-test (all alpha=0.05). Results: Polished samples presented lower roughness (Ra, p=0.015; RSm, p=0.049) and higher SFE (p=0.00). Streptococci had higher CFU in all groups, but the CFU of C albicans was lower for polished samples. Biofilm formation was influenced by the interaction of all factors (p=0.018), and the materials showed no cytotoxicity to FMM-1 growth. Conclusions: Polishing resulted in the lowest values for surface roughness and higher SFE values. Polished ceramics showed less Calbicans adherence while the adherence of Streptococci was greater than C albicans in all conditions. (AU)

• Processo FAPESP: 14/19357-9 - Desempenho de materiais reabilitadores protéticos
• Beneficiário: Marco Antonio Bottino
• Modalidade de apoio: Auxílio à Pesquisa - Regular