Rapid identification of Paracoccidioides lutzii and P-Brasiliensis using Fourier Transform Infrared spectroscopy

Autor(es)

Comparato Filho, Olavo O. ^[1] ; Morais, V, Flavia ; Bhattacharjee, Tanmoy ^[1] ; Castilho, Maiara L. ^[2] ; Raniero, Leandro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Lab Nanossensores, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba, Lab Bionanotecnol, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Molecular Structure; v. 1177, p. 152-159, FEB 5 2019.
• Citações Web of Science: 1

Resumo

The inhalation of the fungus Paracoccidioides brasiliensis (P. brasiliensis, Pb03 and Pb18) causes the Parcoccidioidomycosis. Initially, only P. brasiliensis was thought to be responsible for it. Later the researchers found out that another pathogen: Paracoccidioides lutzii (P. lutzii, Pb01) may also cause the disease. Clinical manifestation and treatment differ based on the causative agent, making it important to identify them correctly. Therefore, this study focusses on distinguishing P. brasiliensis from P. lutzii using Fourier Transform Infrared (FTIR) spectroscopy - a rapid, highly specific and sensitive technique that can identify biochemical composition with minimal sample preparation. We acquired thirty spectra each from pure cultures of P. lutzii, and P. brasiliensis. The FTIR spectra showed the difference in biochemical composition, the major differences being in the polysaccharide and proteins vibrations. Principal Component Linear Discriminant Analysis (PC-LDA) could distinguish the species. We acquired nearly 10 spectra per sample in triplicates, resulting around 30 spectra per group. The analysis of spectra indicates biochemical difference. Multivariate analysis is important to identify spectral differences in specific chosen spectra regions where we can take biological or vibration information. PC-LDA could distinguish Pb01 (P. lutzii) with 96% efficiency and Pb03/Pb18 (P.brasiliensis) with 100% efficiency. Thus, FTIR may contribute to clinical diagnosis of this highly prevalent Latin American disease. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/17404-7 - Terapia fotodinâmica associada a nanossondas para o tratamento do câncer
• Beneficiário: Leandro José Raniero
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/09559-5 - Nanossonda de ouro no diagnóstico do Paracoccidioides brasiliensis
• Beneficiário: Leandro José Raniero
• Modalidade de apoio: Auxílio à Pesquisa - Regular