On the Cooperativity Effect in Watson and Crick and Wobble Pairs for a Halouracil Series and Its Potential Quantitative Application Studied through Surface-Enhanced Raman Spectroscopy

Autor(es)

Mamian-Lopez, Monica B. ^{[1, 2]} ; Temperini, Marcia L. A. ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ ABC, Ctr Nat & Human Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, BR-03178200 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytical Chemistry; v. 90, n. 24, p. 14165-14172, DEC 18 2018.
• Citações Web of Science: 1

Resumo

The nature of the cooperativity effect of hydrogen bonds in Watson and Crick and wobble base pairs formed with thymine, uracil, and its 5-halogenated derivatives (5-fluoro, -chloro, and-bromouracil) has been studied through SERS and by using chemometric tools to process data and extract relevant information. Remarkable differences between the two kinds of pairs were clearly observed, and the behavior correlated to the withdrawing character of different substituents at the 5-position of uracil was verified. Multivariate analyses have also unveiled information about the pair's stability, and a stronger cooperativity effect seems to rule the Watson and Crick pairs when compared to wobble pairs. Defined patterns in the behavior of Watson and Crick pairs allowed the design of an indirect methodology for quantifying 5-bromouracil using a partial least squares (PLS) method with variable selection. Limit of detection (LOD) values of 0.037 and 0.112 mmol L-1 in the absence and presence of structurally similar interferences were reached, while its direct surface-enhanced Raman spectroscopy (SERS) quantification is only possible at similar to 45 mmol L-1. (AU)

• Processo FAPESP: 14/07841-3 - Estudo das flutuações temporais e espectrais nos espectros SERS em regime de uma molécula
• Beneficiário: Monica Benicia Mamian Lopez
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/21070-5 - Espectroscopia vibracional com resolução espacial
• Beneficiário: Mauro Carlos Costa Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Temático