Structural and Functional Characterization of PR-4 SUGARWINs From Sugarcane and Their Role is Plant Defense

Autor(es)

Franco, Flavia P. ^[1] ; Dias, Renata O. ^[1] ; Toyama, Danyelle ^[2] ; Henrique-Silva, Fldvio ^[2] ; Moura, Daniel S. ^[3] ; Silva-Filho, Marcio C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, DE - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 9, JAN 7 2019.
• Citações Web of Science: 1

Resumo

SUGARWIN1 and 2 are defense proteins from sugarcane. Their gene expression is known to be induced in response to wound and Diatraea saccharalis damage. Although the recombinant SUGARWIN protein does not affect insect development, it promotes significant morphological and physiological changes in Fusarium verticillioides and Colletotrichum falcaturn, which lead to fungal cell death via apoptosis. In this study, we deepen our understanding of the role of SUGARWINs in plant defense and the molecular mechanisms by which these proteins affect fungi by elucidating their molecular targets. Our results show that SUGARWINs play an important role in plant defense against opportunistic pathogens. We demonstrated that SUGARWINs are induced by C. falcatum, and the induction of SUGARWINs can vary among sugarcane varieties. The sugarcane variety exhibiting the highest level of SUGARWIN induction exhibited a considerable reduction in C. falcatum infection. Furthermore, SUGARWIN1 exhibited ribonuclease, chitosanase, and chitinase activity, whereas SUGARWIN2 exhibited only chitosanase activity. This variable enzymatic specificity seems to be the result of divergent amino acid composition within the substrate-binding site. (AU)

• Processo FAPESP: 14/50275-9 - Decifrando os mecanismos moleculares envolvidos na localização subcelular de proteínas e na complexa interação planta-insetos-patógenos
• Beneficiário: Márcio de Castro Silva Filho
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 13/12577-0 - Estudo das bases moleculares da ação da SUGARWIN em fungos fitopatogênicos
• Beneficiário: Flávia Pereira Franco
• Modalidade de apoio: Bolsas no Brasil - Doutorado