Microsatellite Markers Transferability in Culex nigripalpus (Diptera: Culicidae)

Autor(es)

de Carvalho, Gabriela Cristina ^[1] ; Marrelli, Mauro Toledo ^[2] ; Bruno Wilke, Andre Barretto ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, Ave Doutor Arnaldo 715, BR-01246904 Sao Paulo, SP - Brazil

^[2] Univ Miami, Miller Sch Med, Dept Publ Hlth Sci, 1120 Northwest 14th St, Miami, FL 33136 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Entomology; v. 55, n. 5, p. 1334-1336, SEP 2018.
• Citações Web of Science: 2

Resumo

Microsatellite loci specifically designed and optimized for secondary vectors and neglected species of mosquitoes are lacking. Cross-species transferability of microsatellite loci between close taxa has been used as a reliable option, being successfully done for Aedes taeniorhynchus Wiedemann (Diptera: Culicidae), Triatoma sordida Stal (Hemiptera: Reduviidae), and Aedes fluviatilis Lutz (Diptera: Culicidae). Nonetheless, prior to the utilization of transferred microsatellites, they must be assessed for their performance and reliability on the target species. Therefore, the goal of this study was to transfer and validate SSR primers previously developed for the Culex pipiens complex to Culex (Culex) nigripalpus Theobald (Diptera: Culicidae). Culex nigripalpus were collected in seven different locations throughout the city of Sao Paulo using CDC traps baited with dry ice between 2011 and 2013, yielding a total of 223 specimens. Six SSR primers were successfully transferred to Cx. nigripalpus yielding satisfactory results for allele richness, ranging from 5 to 12 alleles per locus. The expected heterozygosity values were higher than the observed heterozygosity (P < 0.05) for the loci CxqCTG10, CxqGT51, and Cxpq59. Linkage disequilibrium results were statistically significant only for the comparison between the loci CxqCA115 and CxqCTG10, and the estimation for the probability of null alleles was also low, ranging from 0 to 0.052. These results substantially validate the SSRs transference to Cx. nigripalpus as well as their subsequent use in microevolution studies comprising this species. (AU)

• Processo FAPESP: 13/18965-2 - Análise da estrutura populacional de Mosquitos Culex quinquefasciatus e Culex nigripalpus (Diptera: Culicidae) utilizando microssatélites e análise de morfometria de asas
• Beneficiário: Gabriela Cristina de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/15313-4 - Análise da estrutura populacional de mosquitos (Diptera: Culicidae) utilizando marcadores microssatélites e morfometria alar
• Beneficiário: Mauro Toledo Marrelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular