Synthesis and application of triclosan methacrylate monomer in resin composites

Autor(es)

de Paula, Andreia Bolzan ^[1] ; Taparelli, Jesus Roberto ^[2] ; Bruschi Alonso, Roberta Caroline ^{[3, 4]} ; Innocentini-Mei, Lucia Helena ^[2] ; Puppin-Rontani, Regina M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Dent Mat Div, Limeira Ave 901, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Sch Chem Engn, Dept Mat & Bioproc Engn, Campinas, SP - Brazil

^[3] Mogi das Cruzes Univ UMC, Technol & Res Ctr NPT, Mogi Das Cruzes, SP - Brazil

^[4] Metropolitan Univ Santos UNIMES, Sch Dent, Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 23, n. 2, p. 965-974, FEB 2019.
• Citações Web of Science: 2

Resumo

ObjectivesTo evaluate the antibacterial activity, bacterial viability, cytotoxicity, and mechanical/physical properties of a novel methacrylate triclosan-derivative monomer (TM) incorporated in dental resin composite.MethodsTM was synthesized by esterification and, after characterization by FT-IR, was added to an experimental composite. Samples were divided into two groups according to TM presence, i.e., C1 (control) and C2 (C1 + 14.4% TM). Microbiological properties: Specimens (C1 and C2) were prepared and placed on bacterial suspensions of Streptococcus mutans. Antibacterial activity, MTT, and live/dead bacterial viability were used to test the resin composites. All assays were performed in triplicates. Mechanical properties: Specimens underwent compression (CS) and flexural strength (FS) tests conducted in an Instron universal testing machine at a crosshead speed of 0.5mm/min. Physical properties: Specimens were assessed for Knoop hardness (KHN) and crosslink density (CD). Fourier transform infrared spectroscopy allowed the degree of conversion (DC) to be evaluated. Data were subjected to appropriate statistical tests according to data distribution and assay (p<0.05).ResultsMicrobiological properties: C2 showed the lowest biofilm accumulation and the highest membrane-compromised bacteria in the biofilm. Mechanical/physical properties: For CS, FS, KHN, and DC, there was no significant difference between groups C1 and C2; however, significant difference was observed for the CD assay.ConclusionsThe triclosan methacrylate reduces bacterial adhesion of S. mutans and decreased the formation of bacterial biofilm without affecting important polymer properties. The triclosan methacrylate incorporated in resin composite could greatly reduce the live bacterial adhesion of S. mutans and decrease the formation of bacterial biofilm without affecting important polymer properties.Clinical significanceThe resin composites containing triclosan methacrylate could greatly reduce the bacterial adhesion and biofilm formation. That might prevent the secondary caries round the margins of the restorations. (AU)

• Processo FAPESP: 12/10750-4 - Influência da incorporação de um monômero antimicrobiano a base de triclosan, em um compósito resinoso experimental, na resistência à biodegradação e sobre o biofilme cariogênico
• Beneficiário: Regina Maria Puppin Rontani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/14151-5 - Influência da incorporação de um monômero antimicrobiano a base de Triclosan, em um compósito resinoso experimental, na resistência à biodegradação e sobre o biofilme cariogênico.
• Beneficiário: Andréia Bolzan de Paula
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado