Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion

Autor(es):Mostrar menos -

Galasso, Marcos ^[1] ; Feld, Jordan J. ^[2] ; Watanabe, Yui ^[1] ; Pipkin, Mauricio ^[1] ; Summers, Cara ^[1] ; Ali, Aadil ^[1] ; Qaqish, Robert ^[1] ; Chen, Manyin ^[1] ; Ribeiro, Rafaela V. P. ^[1] ; Ramadan, Khaled ^[1] ; Pires, Layla ^[1] ; Bagnato, Vanderlei S. ^[3] ; Kurachi, Cristina ^[3] ; Cherepanov, Vera ^[2] ; Moonen, Gray ^[1] ; Gazzalle, Anajara ^[1] ; Waddell, Thomas K. ^[1] ; Liu, Mingyao ^[1] ; Keshavjee, Shaf ^[1] ; Wilson, Brian C. ^[4] ; Humar, Atul ^[5] ; Cypel, Marcelo ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Hlth Network, Toronto Gen Res Inst, Latner Thorac Surg Res Labs, Toronto, ON M5G 2C4 - Canada

^[2] Univ Hlth Network, Toronto Gen Hosp, Toronto Ctr Liver Dis, Toronto, ON M5G 2C4 - Canada

^[3] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Paulo - Brazil

^[4] Univ Toronto, Dept Med Biophys, Princess Margaret Canc Ctr, Toronto, ON M5G 2C4 - Canada

^[5] Univ Hlth Network, Multiorgan Transplant Program, Toronto, ON M5G 2C4 - Canada

• Tipo de documento: Artigo Científico
• Fonte: NATURE COMMUNICATIONS; v. 10, JAN 29 2019.
• Citações Web of Science: 12

Resumo

Availability of organs is a limiting factor for lung transplantation, leading to substantial mortality rates on the wait list. Use of organs from donors with transmissible viral infections, such as hepatitis C virus (HCV), would increase organ donation, but these organs are generally not offered for transplantation due to a high risk of transmission. Here, we develop a method for treatment of HCV-infected human donor lungs that prevents HCV transmission. Physical viral clearance in combination with germicidal light-based therapies during normothermic ex-vivo Lung Perfusion (EVLP), a method for assessment and treatment of injured donor lungs, inactivates HCV virus in a short period of time. Such treatment is shown to be safe using a large animal EVLP-to-lung transplantation model. This strategy of treating viral infection in a donor organ during preservation could significantly increase the availability of organs for transplantation and encourages further clinical development. (AU)

• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs