RETRACTED: Bovine Herpesvirus 5 promotes mitochondrial dysfunction in cultured bovine monocyte-derived macrophages and not affect virus replication (Retracted article. See vol. 235, pg. 327, 2019)

Autor(es)

Cardoso, Tereza C. ^[1] ; Okamura, Lucas H. ^[1] ; Baptistella, Jamila C. ^[1] ; Borsanelli, Ana Carolina ^[1] ; Baptistiolli, Lillian ^[2] ; Ferreira, Helena L. ^[2] ; Gameiro, Roberto ^[1] ; Flores, Eduardo F. ^[3]

Afiliação do(s) autor(es)

^[1] UNESP Univ Sao Paulo State, Coll Vet Med, Aracatuba, SP - Brazil

^[2] Univ Sao Paulo, Dept Vet Med, FZEA, Pirassununga, SP - Brazil

^[3] Univ Fed Santa Maria, Virol Sect, Dept Prevent Vet Med, Santa Maria, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Microbiology; v. 229, p. 153-158, FEB 2019.
• Citações Web of Science: 1

Resumo

Bovine alphaherpesvirus 1 (BHV1) and 5 (BHV5) are known to establish latent infections in sensory neurons of the trigeminal ganglion, yet leukocytes and tonsils have also been described as sites of latency in experimentally infected cattle. Little information is available on which immune cells are susceptible to BHV5 infection and how the infection may affect cell bioenergy. The aim of this study was to determine whether primary bovine monocyte-derived macrophages are susceptible to BHV5 infection and to evaluate parameters such as cell survival, virus replication and nitric oxide (NO) production. In addition, production of reactive oxygen species and mitochondrial damage were also analyzed. BHV5 infected cells underwent low rates of apoptosis but activated mitochondriat membrane depolarization and complex I. Additionally, production of high NO levels upon monocyte derived-macrophage infection did not interfere with the production of progeny virus. Overall, our findings revealed that primary cultures of bovine monocyte-derived macrophages support BHV5 replication in vitro and that mitochondrial dysfunction induced by infection apparently does not interfere with virus replication. (AU)

• Processo FAPESP: 12/16715-6 - Herpesvirus bovino tipo 5: expressão de genes relacionados à morte celular programada em infecção in vitro
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular