Evaluation of genetic polymorphisms in MMP2, MMP9 and MMP20 in Brazilian children with dental fluorosis

Autor(es):Mostrar menos -

Romualdo, Priscilla Coutinho ^[1] ; Pucinelli, Carolina Maschietto ^[1] ; Tannure, Patricia Nivoloni ^[2] ; Nelson-Filho, Paulo ^[1] ; Bezerra Segato, Raquel Assed ^[1] ; Brancher, Joao Armando ^[3] ; Magalhaes, Nilza Leticia ^[1] ; Costa, Marcelo de Castro ^[4] ; Antunes, Livia Azeredo A. ^[5] ; Antunes, Leonardo Santos ^[5] ; Rabelo Buzalaf, Marilia Afonso ^[6] ; Charone, Senda ^[6] ; Kuchler, Erika Calvano ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Dent Sch, Dept Pediat Dent, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Veiga de Almeida, Sch Dent, Rio De Janeiro, RJ - Brazil

^[3] Univ Posit, Dept Dent, Curitiba, Parana - Brazil

^[4] Univ Fed Rio de Janeiro, Sch Dent, Dept Pediat Dent & Orthodont, Rio De Janeiro, RJ - Brazil

^[5] Fluminense Fed Univ, Sch Dent, Nova Friburgo, RJ - Brazil

^[6] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY; v. 66, p. 104-108, FEB 2019.
• Citações Web of Science: 1

Resumo

Recent studies suggested that genetics contribute to differences in dental fluorosis (DF) susceptibility among individuals having the same environmental exposure. This study evaluated if MMP2, MMP9 and MMP20 are expressed during enamel development and assessed the association between polymorphisms in these genes with DF. Mice susceptible and resistant to DF were used to evaluate if MMPs were candidate genes for DF. The animals received fluoride and their enamels were used for immunohistochemistry. Additionally, 481 subjects from a city with fluoridation of public water supplies were recruited. Genotyping was performed using real time PCR. Allele/genotype frequencies were compared between groups. MMP2, MMP9 and MMP20 immunostaining was detected in both animal groups. DF was observed in 22.4% of the subjects. A borderline association was observed in MMP2 (rs243865), MMP9 (rs17576) and in MMP20 (rs1784418) (p = 0.06, p = 0.08 and p = 0.06 respectively). Briefly, MMPs were expressed during enamel maturation and genetic polymorphisms were not associated with DF. (AU)

• Processo FAPESP: 15/06866-5 - Avaliação do papel do estrógeno no desenvolvimento dentofacial
• Beneficiário: Erika Calvano Kuchler
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores