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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genetic Mapping With Allele Dosage Information in Tetraploid Urochloa decumbens (Stapf) R. D. Webster Reveals Insights Into Spittlebug (Notozulia entreriana Berg) Resistance

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Autor(es):
Ulbricht Ferreira, Rebecca Caroline [1] ; de Castro Lara, Leticia Aparecida [2] ; Chiari, Lucimara [3] ; Lima Barrios, Sanzio Carvalho [3] ; do Valle, Cacilda Borges [3] ; Valerio, Jose Raul [3] ; Vilela Torres, Fabricia Zimermann [3] ; Franco Garcia, Antonio Augusto [2] ; de Souza, Anete Pereira [1, 4]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Ctr Mol Biol & Genet Engn, Campinas, SP - Brazil
[2] Univ Sao Paulo, Genet Dept, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil
[3] Brazilian Agr Res Corp, Ernbrapa Beef Cattle, Campo Grande - Brazil
[4] Univ Estadual Campinas, Plant Biol Dept, Biol Inst, Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN PLANT SCIENCE; v. 10, FEB 21 2019.
Citações Web of Science: 2
Resumo

Urochloa decumbens (Stapf) R. D. Webster is one of the most important African forage grasses in Brazilian beef production. Currently available genetic-genomic resources for this species are restricted mainly due to polyploidy and apomixis. Therefore, crucial genomic-molecular studies such as the construction of genetic maps and the mapping of quantitative trait loci (QTLs) are very challenging and consequently affect the advancement of molecular breeding. The objectives of this work were to (i) construct an integrated U. decumbens genetic map for a full-sibling progeny using GBS-based markers with allele dosage information, (ii) detect QTLs for spittlebug (Notozulia entreriana) resistance, and (iii) seek putative candidate genes involved in defense against biotic stresses. We used the Setaria viridis genome a reference to align GBS reads and selected 4,240 high-quality SNP markers with allele dosage information. Of these markers, 1,000 were distributed throughout nine homologous groups with a cumulative map length of 1,335.09 cM and an average marker density of 1.33 cM. We detected QTLs for resistance to spittlebug, an important pasture insect pest, that explained between 4.66 and 6.24% of the phenotypic variation. These QTLs are in regions containing putative candidate genes related to defense against biotic stresses. Because this is the first genetic map with SNP autotetraploid dosage data and QTL detection in U. decumbens, it will be useful for future evolutionary studies, genome assembly, and other QTL analyses in Urochloa spp. Moreover, the results might facilitate the isolation of spittlebug-related candidate genes and help clarify the mechanism of spittlebug resistance. These approaches will improve selection efficiency and accuracy in U. decumbens molecular breeding and shorten the breeding cycle. (AU)

Processo FAPESP: 08/52197-4 - Genomic-assisted breeding of sugarcane: using molecular markers for understanding the genetic architecture of quantitative traits and to implement marker assisted selection
Beneficiário:Anete Pereira de Souza
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 05/51010-0 - Desenvolvimento e caracterização de marcadores microssatélites em espécies forrageiras tropicais para utilização em estudos genéticos e melhoramento
Beneficiário:Anete Pereira de Souza
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/19219-6 - Detecção de genes de interesse agronômico e envolvidos na heterose em Urochloa spp
Beneficiário:Rebecca Caroline Ulbricht Ferreira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado