Seminal fluid production and sperm packaging in dromiid crabs (Brachyura, Podotremata)

Autor(es)

Garcia Bento, Maria Alice ^{[1, 2]} ; Lopez Greco, Laura S. ^[3] ; Zara, Fernando Jose ^{[1, 2, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, FCAV, Lab Morfol Invertebrados IML, Dept Biol Aplicada A, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Ctr Aquiculutra, CAUNESP, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[3] Univ Buenos Aires, CONICET, IBBEA, Lab Biol Reprod & Crecimiento Crustaceos De, Fac Ciencias Exactas & Nat, Dept Biodiversidad & B, C1428EGA, Buenos Aires, DF - Argentina

^[4] Univ Estadual Paulista, UNESP, IB, Ave 24 A, 1515, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZOOLOGY; v. 132, p. 17-30, FEB 2019.
• Citações Web of Science: 2

Resumo

Reproductive anatomy, including sperm storage structures and sperm transfer, is an important feature used to analyze phylogenetic relationships among taxa. We describe the male reproductive anatomy, seminal fluid production and packaging of spermatozoa in the vas deferens of primitive crabs. In all species of Dromiidae, the testes were tubular type and the vas deferens is a tube with a simple epithelium. The spermatozoa are in a central mass immersed in type I secretion, forming a large spermatic cord. In Moreiradromia antillensis and Dromia erythropus the spermatic cord is surrounded by a more complex secretion layer composed by protein granules and polysaccharides that seem to be responsible to form the sperm plaque which was absent in Hypoconcha. The sperm plaque was found in all Dromiinae species, which may result from the mating and probably act as a barrier to subsequent copulations. These patterns of elongated coenospermic spermatophore found here are unique and different from the spermatophores of all true crabs and may be considered a plesiomorphic state to spermatophoric structure in Brachyura. Our results show a novel type of spermatozoa packaging and new insights into how sperm might be transferred in Podotremata. (AU)

• Processo FAPESP: 16/10394-4 - Sistema reprodutor masculino e espermiotaxonomia em Dromiidae: existe um só padrão de produção de fluido seminal, empacotamento do espermatozoide e transferência espermática em caranguejos?
• Beneficiário: Maria Alice Garcia Bento
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/21294-5 - Sistema reprodutor em Dromiidae: caracterização histoquímica e ultraestrutural dos espermatozóides e fluido seminal em Hypoconcha parasitica (Linnaeus, 1763)
• Beneficiário: Maria Alice Garcia Bento
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/50188-8 - Crustáceos decápodes: multidisciplinaridade na caracterização da biodiversidade marinha do estado de São Paulo (taxonomia, espermiotaxonomia, biologia molecular e dinâmica populacional) (Biodiversidade Marinha)
• Beneficiário: Fernando Luis Medina Mantelatto
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático