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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

HYPERTONIC SALINE SOLUTION REDUCES MICROCIRCULATORY DYSFUNCTION AND INFLAMMATION IN A RAT MODEL OF BRAIN DEATH

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Autor(es):
Correia, Cristiano de Jesus [1] ; Armstrong, Jr., Roberto [1] ; de Carvalho, Priscila Oliveira [1] ; Simas, Rafael [1] ; Janolli Sanchez, Daniela Crisina [1] ; Breithaupt-Faloppa, Ana Cristina [1] ; Sannomiya, Paulina [1] ; Pinho Moreira, Luiz Felipe [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Inst Coracao Incor, Lab Cirurg Pesquisa Cardiovasc, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Shock; v. 51, n. 4, p. 495-501, APR 2019.
Citações Web of Science: 2
Resumo

Background: Brain death (BD) induces hemodynamic instability with microcirculatory hypoperfusion, leading to increased organ inflammation and dysfunction. This study investigated the effects of 7.5% hypertonic saline solution (HSS) on mesenteric microcirculatory dysfunction and inflammation in a rat model of BD. Methods: Male Wistar rats were anesthetized and mechanically ventilated. BD was induced by rapidly inflating an intracranial balloon catheter. The rats were randomly divided into: SH, sham-operated rats subjected to trepanation; NS, rats treated with NaCl 0.9%, 4 mL/kg immediately after BD; T-1, rats treated with HSS (NaCl 7.5%, 4 mL/kg) immediately or 60 min after BD, T-60. All groups were analyzed 180 min after the start of the experiment. Results: Rats in BD groups presented with a similar hypertensive peak, followed by hypotension. Proportion of perfused small vessels was decreased in the NS group (46%) compared with the SH group (74%, P = 0.0039). HSS restored the proportion of perfused vessels (T-1 = 71%, P = 0.0018). The anti-endothelial nitric oxide synthase (eNOS) protein expression significantly increased in rats given HSS (T-1, and T-60, P = 0.0002). Similar results were observed regarding endothelin-1 (P<0.0001). Increased numbers of rolling (P = 0.0015) and migrated (P = 0.0063) leukocytes were observed in the NS group compared with the SH group. Rats given HSS demonstrated an overall reduction in leukocyte-endothelial interactions. The ICAM-1 levels increased in the NS group compared with the SH group, and decreased in the HSS-treated groups (P = 0.0002). Conclusions: HSS may improve the density of mesenteric perfused small vessels due to its effects on eNOS and endothelin-1 protein expression, and reduces inflammation by decreasing leukocyte adhesion and migration in a rat model of BD. (AU)

Processo FAPESP: 12/19841-2 - Estudo dos efeitos da solução salina hipertônica sobre as alterações microcirculatórias, do tecido cardíaco e desenvolvimento do processo inflamatório em modelo de morte encefálica em ratos
Beneficiário:Luiz Felipe Pinho Moreira
Linha de fomento: Auxílio à Pesquisa - Regular